FIGURE

Fig. 2

ID

ZDB-FIG-080701-31

Publication

Mara et al., 2008 - Two deltaC splice-variants have distinct signaling abilities during somitogenesis and midline patterning

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Fig. 2

deltaC^tv2 does not signal effectively during somitogenesis. (A) qPCR showing the relative abundance of deltaC^tv2 in 15 somite embryos of various genetic backgrounds. Levels of deltaC^tv2 increase significantly in morpholino (mo) injected embryos. The amount of deltaC^tv2 mRNA is graphed as a percentage of total deltaC mRNA. (B–C) titin staining marks the somite boundaries of deltaC^+/- embryos. The first disrupted somite border in a morpholino injected embryo is indicated by the arrow in (C). (D) The average number of somites formed in each experimental condition. n = 93, 102, 73, 115 from top to bottom. (E–F) The onset of border defects in deltaD^-/- embryos is shifted anteriorly in morpholino injected embryos. Arrows indicate the first abnormal border. (G) The average number of somites formed in each experimental condition. n = 73, 91, 84, 139, 99 from top to bottom. Two different sets of mutant and sibling embryos were used for the experiments marked in red and yellow. Error bars indicate standard deviation.

Expression Data

Gene:	ttn.2
Fish:	WT dld^tg249/tg249 WT + MO7-dlc + MO8-dlc dld^tg249/tg249 + MO7-dlc + MO8-dlc
Knockdown Reagents:	MO7-dlc MO8-dlc
Anatomical Term:	somite
Stage:	26+ somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO7-dlc + MO8-dlc dld^tg249/tg249 + MO7-dlc + MO8-dlc
Knockdown Reagents:	MO7-dlc MO8-dlc
Observed In:	somite border
Stage:	26+ somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 318(1), Mara, A., Schroeder, J., and Holley, S.A., Two deltaC splice-variants have distinct signaling abilities during somitogenesis and midline patterning, 126-132, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.