FIGURE

Fig. 6

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ZDB-FIG-080604-84

Publication

Sidi et al., 2008 - Chk1 Suppresses a Caspase-2 Apoptotic Response to DNA Damage that Bypasses p53, Bcl-2, and Caspase-3

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Fig. 6

Influence of Genetic Background on Gö6976-Mediated Radiosensitization of Human Cancer Cells

(A) Western blots comparing the levels of caspase-2 (pro and cleaved forms) and cleaved caspase-3 in 24-hpIR lysates from TP53^+/+ and TP53^-/- HCT116 cells that were treated with or without IR (10 Gy) or Gö6976 (1 μM).

(B) Analysis as in (A) of the SAOS2 (left), MDA-MB-435 (middle), and LN-428 (right) lines.

(C) Apoptotic cell numbers at 48 hpIR as measured by Annexin V (+) / PI (-) staining of the indicated cell lines treated with 0 or 10 Gy IR with or without Gö6976 (1 μM). Data are means ± SEM. See Figure S9 for a CHK1 shRNA-mediated phenocopy of Gö6976 in TP53^-/- HCT116 cells.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Cell, 133(5), Sidi, S., Sanda, T., Kennedy, R.D., Hagen, A.T., Jette, C.A., Hoffmans, R., Pascual, J., Imamura, S., Kishi, S., Amatruda, J.F., Kanki, J.P., Green, D.R., D'Andrea, A.A., and Look, A.T., Chk1 Suppresses a Caspase-2 Apoptotic Response to DNA Damage that Bypasses p53, Bcl-2, and Caspase-3, 864-877, Copyright (2008) with permission from Elsevier. Full text @ Cell