FIGURE

Fig. 8

ID

ZDB-FIG-080604-44

Publication

Chi et al., 2008 - Genetic and Physiologic Dissection of the Vertebrate Cardiac Conduction System

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Fig. 8

s634 Affects tcf2
(A) Lateral views of brightfield images of wild-type, s634 mutant, and 0.5 ng tcf2 ATG MO-injected embryos at 48 hpf, anterior to the left. Despite wild-type cardiac morphology, s634 mutants and tcf2 MO- injected hearts exhibit an AV conduction block, resulting in reduced cardiac output and pronounced pericardial edema (arrow).

(B) Genetic map of the s634 region. Numbers below simple sequence length polymorphism markers indicate recombination events. Two genes were identified within the critical region, which spans two bacterial artificial chromosomes.

(C) Sequencing of tcf2^s634 cDNA revealed a G to A change at base pair 522, resulting in a premature stop codon at amino acid 174 thereby removing the homeobox and transactivation domains.

(D) Whole mount RNA in situ hybridization at 48 hpf reveals tcf2 expression at the AV canal (arrowhead) and the OFT (arrow) region of the ventricle.

(E) Injection of wild-type tcf2 mRNA rescued the heart phenotype of ∼82% of s634 mutants, while injection of mutant tcf2 mRNA failed to rescue.

A, atrium; V, ventricle.

Expression Data

Gene:	hnf1ba
Fish:	WT
Anatomical Terms:	atrioventricular canal bulbus arteriosus cardiac ventricle
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hnf1ba^s917/s917
Observed In:	heart looping pericardium regulation of cardiac muscle contraction
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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