FIGURE

Fig. 3

ID

ZDB-FIG-080529-66

Publication

Marin et al., 2003 - A noncanonical sequence phosphorylated by casein kinase 1 in {beta}-catenin may play a role in casein kinase 1 targeting of important signaling proteins

Other Figures

Fig. 3

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Fig. 3

The phosphorylation of full-length β-catenin WT and mutants by CK1α. The recombinant, bacterially expressed, (His)₆-tagged α-catenin WT and mutants from zebrafish were phosphorylated in vitro with the homologous CK1αL enzyme as described in Materials and Methods. The mutants included Ser-45 replaced by alanine (S45A), Leu-46 replaced by alanine (L46A), and all components of the acidic cluster (E53, D54, E55, D56, and D58) changed to alanine (AC/A). (Upper) Bands are autoradiography of the ³²P incorporated into β-catenin after SDS/PAGE fractionation. (Lower) Band represents the Western blot of the (His)₆ β-catenin present that was developed with monoclonal anti-(His)₆ antibody.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA