pax6a and pax6b Expression Analysis by Wholemount RNA in situ Hybridisation during Early Zebrafish Development pax6a expression is shown in (A) and (C) at 24 hpf; (A) lateral view showing expression in eye, telencephalon, diencephalon, hind brain, and neural tube (C) dorsal view; (E) and (G) lateral and dorsal view at 32 hpf, showing continuing pattern of expression, (I) Vibratome coronal section of wholemount stained embryo expressing pax6a in telencephalon (arrowed), diencephalon, retina and lens. (B) pax6b expression is seen in lateral view showing eye and optic tectum at 24 hpf, pancreas expression is highlighted using black triangles; (D) dorsal view at 24 hpf. (F) and (H) lateral and dorsal. Increasing hindbrain and neural tube expression of pax6b is revealed by 32 hpf embryos. (J) predominantly eye expression of pa6b is seen in coronal section at 32 hpf.
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