Fig. 6

The diwanka locus maps on LG 23 and is deleted in b531 mutant embryos. (A) PCR amplification using marker z1403 and DNA isolated from the G₀ diwanka Tü fish, the G₀ WIK fish, pooled F₂ mutants, and pooled F₂ siblings. The Tü-specific PCR fragment is shorter than the WIK-specific fragment. Amplification with z1403 in the F₂ sibling pool DNA results in a Tü-specific and a WIK-specific fragment, while in DNA from pooled mutant only the Tü-specific fragment is present (higher mobility), indicating linkage between z1403 and the diwanka mutation. (B) PCR amplification using marker z1403 and DNA from individual F2 sibling embryos (top). (Bottom) PCR amplification using marker z1403 and DNA from individual F₂ diwanka mutant embryos. In a recombinant embryo, a Tü-specific and a WIK-specific fragment are present (*). (C) Schematic representation of LG 23 around the diwanka locus. A black circle indicates the centromere. (D) PCR amplification of SSLP markers from DNA of four b531/b531 mutant embryos and four sibling embryos. In sibling embryos (sibs), all tested markers are present. In the four b531/b531 embryos, marker z10949 is present, while markers z9351 and z7243 are absent. (E) Schematic representation of LG 23 in b531/b531 embryos. In these embryos, LG 23 breaks between z10949 and z9351, deleting the remaining chromosomal arm, including the diwanka locus.