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Fig. 3

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ZDB-FIG-080429-26

Publication

Rastegar et al., 2002 - A floor plate enhancer of the zebrafish netrin1 gene requires Cyclops (Nodal) signalling and the winged helix transcription factor FoxA2

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Fig. 3

Stable expression of the –1.1netrin1:gfp/FPE transgene. (A) Expression of the transgene in floor plate (f) and hypochord (h) of a 32-hpf embryo. (B, C) Whole-mount in situ hybridisation of transgenic embryos with gfp antisense RNA at the 32-hpf (B) and one-somite stages (C). (D, E) Comparison of expression of netrin1 (D) and gfp (E) mRNA in the head of 24-hpf transgenic embryos. Note that, unlike netrin1 (D), the transgene (E) is not expressed in the ventral forebrain of embryos. (A, B, D, E) Lateral views, anterior left and dorsal up. (C) Dorsal view, anterior up. Abbreviations: f, floor plate; h, hypochord. Scale bar represents 50 μm (A, B, D, E) and 100 μm (C).

Expression Data

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Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 252(1), Rastegar, S., Albert, S., Le Roux, I., Fischer, N., Blader, P., Müller, F., and Strähle, U., A floor plate enhancer of the zebrafish netrin1 gene requires Cyclops (Nodal) signalling and the winged helix transcription factor FoxA2, 1-14, Copyright (2002) with permission from Elsevier. Full text @ Dev. Biol.