FIGURE

Fig. 4

ID

ZDB-FIG-080411-27

Publication

Pagnon-Minot et al., 2008 - Collagen XV, a novel factor in zebrafish notochord differentiation and muscle development

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Fig. 4

48 hpf embryos injected with anti-sense Col15a1 morpholinos (B) are almost similar to the embryos injected with Col15a1-Ms (A) except they are slightly shorter. Histological analysis of collagen XV morphants. Transversal sections of wild-type (C), Col15a1-Ms (D) and Col15a1-MO injected embryos at 48hpf. In Col15a1-MO, the peri-notochordal sheath is thinner compared to wild-type and control embryos. In 48 hpf collagen XV morphants, the muscular tissue is disorganized and horizontal myosepta are no visible. Neural tube and vessels are normal. (F, G) Lateral views of wild-type embryos (F) and Col15a1-MO embryos (G) at 48 hpf stained with antibodies to dystrophin to reveal the shape of myotomes. Contrary to wild type embryos (F), morphants (G) exhibit a U-shape of the myotomes. Magnification: A: x 200; B: x 200; C–E: x 400; F, G: x 300.

Expression Data

Gene:	dmd
Fish:	WT WT + MO1-col15a1a
Knockdown Reagent:	MO1-col15a1a
Anatomical Term:	myotome
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-col15a1a
Knockdown Reagent:	MO1-col15a1a
Observed In:	blood vasculature horizontal myoseptum muscle myotome perichordal connective tissue
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 316(1), Pagnon-Minot, A., Malbouyres, M., Haftek-Terreau, Z., Kim, H.R., Sasaki, T., Thisse, C., Thisse, B., Ingham, P.W., Ruggiero, F., and Le Guellec, D., Collagen XV, a novel factor in zebrafish notochord differentiation and muscle development, 21-35, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.