Fig. 6

nok and aPKCi are not sufficient to tether each other within the nucleus. Images are reconstructions of confocal Z-stack sections imaged on late gastrula stage whole mounts. Myc and aPKC stainings are false-colored. A,B: Embryos expressing high levels of HisMyc-tagged nok^ΔNES were counterstained with anti aPKC. A,A′: Within DL cells, high levels of nuclear HisMyc-tagged nok^ΔNES are present whereas aPKC localizes to outer cell membranes and no increased levels of nuclear aPKC are detectable (A“). B,B′: Within epithelial EVL cells, HisMyc-tagged nok^ΔNES is at outer cell membranes and within the nucleus. B”: aPKC is predominantly associated with outer cell membranes. C: Embryos overexpressing HisMyc-tagged nok^wt (green) and non-tagged aPKCi^KD (red), whereas nok^wt is absent from the nucleus (C′), aPKCi^KD is nuclear. All images are apical views onto the tissue.