FIGURE

Fig. 4

ID

ZDB-FIG-080326-61

Publication

Pézeron et al., 2008 - Rasl11b knock down in zebrafish suppresses one-eyed-pinhead mutant phenotype

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Fig. 4

rasl11b knock down induces a specific curly down phenotype in zebrafish. (A) Scheme of the rasl11b transcript. The Morpholino targeted sequences are represented by thin red lines. The red boxes correspond to the G1 to G5 domains described in Fig. 1. (B–D) Lateral view of 1 dpf embryos. Both rasl11b MO-ATG and rasl11b MO-GT induce a curly tail down phenotype when injected in WT. This phenotype can be rescued by the coinjection of rasl11b mRNA resistant to MO knock down, rasl11b^MOr (D). (E) In vitro translational assay showing the efficient knock down effect of the rasl11b MO-ATG on wild-type rasl11b mRNA compared to the MO resistant rasl11b^MOr mRNA. (F,G) rasl11b splice-blocking morpholino (rasl11b MO-GT) knocks down endogenous rasl11b mRNA. RT-PCR analysis detected two splice variants that were cloned and sequenced. Sequence comparison revealed that the splice variant (1) resulted from aberrant splicing to an upstream cryptic slice donor site twn endogenous rasl11b mRNA. RT-PCR analysis detected two splice variants that were cloned and sequenced. Sequence comparison revealehat is present in intron 2, r

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-rasl11b WT + MO2-rasl11b
Knockdown Reagents:	MO1-rasl11b MO2-rasl11b
Observed In:	post-vent region
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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