Fig. 7

BMP Beads Direct Cardiac Jogging (A and B) Phospho-Smad 1,5,8 antibody staining after implantation at the 15-somite stage of a PBS bead (A) or a BMP bead (B). Bracket in (B) indicates the induced phospho-Smad staining in close proximity of the BMP bead. Dorsal view with anterior to the top of 25-somite stage embryos. (C–E) ISH with antisense cmlc2 riboprobe of embryos injected with a bmp4 MO and a BMP bead implanted at the 15–18-somite stage. (C) The heart tube is redirected to a BMP bead implanted on the right side (11/15), dorsal view with anterior to the top, arrow indicates position of the BMP bead. (D) In a lateral view, arrows indicate CPCs that have been recruited toward the BMP bead (n = 7/8). Arrowheads (E) point to a layer of CPCs that form a layer on the bead's surface. (E) is an enlargement of (D). (F–H′) Selected images of a confocal time-lapse recording of bmp4 morphants (F–G′) or a bmp4 has2 double morphant (H and H′) with a BMP bead placed on the right side. Images represent the start (F–H) and end point (F′,G′,H′) of the time-lapse of the three individual embryos. Position of the BMP beads is marked by the blue circle.