Fig. 2
- ID
- ZDB-FIG-080325-59
- Publication
- Wilkins et al., 2008 - Mtx2 directs zebrafish morphogenetic movements during epiboly by regulating microfilament formation
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YSL nuclei are present but disordered in mtx2 morphants. (A–H) Fluorescence microscope images of SYTOX Green injected embryos. Wild-type (A–C) and mtx2 MO (D–F) injected embryos (two- to four-cell stage), injected at 1000-cell stage with SYTOX Green. (A, D) Arrowheads indicate YSN at the blastoderm margin. mtx2 MO injected embryos have a similar collection of YSN at the blastoderm margin (arrowheads in panel D) but show an abnormal distribution of YSN in the inner YSL (I-YSL), which persists (E) until just before yolk cell lysis (F) when the YSN disintegrate. High magnification, merged bright field/fluorescence imaging (G, H) shows the majority of I-YSL nuclei are evenly spaced in wild-type embryos (G) but randomly distributed in mtx2 MO injected embryos (H). Scale bar = 100 μm in panel F (applies to panels A–F), panels G and H. (I) YSN spatial distribution analysis. At distances beyond the diameter of each YSL nucleus (shaded red zone), wild-type YSN are dispersed with maximal deflection from complete spatial randomness (CSR) at r = 22 μm (asterisk indicates significance p value < 0.001). However, in mtx2 morphants, the nuclei are randomly distributed. K-functions are means for wild-type (n = 10) and mtx2 MO (n = 6) embryos, standardized on the 99% confidence interval (CI). |
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| Stage Range: | Shield to Bud |
Reprinted from Developmental Biology, 314(1), Wilkins, S.J., Yoong, S., Verkade, H., Mizoguchi, T., Plowman, S.J., Hancock, J.F., Kikuchi, Y., Heath, J.K., and Perkins, A.C., Mtx2 directs zebrafish morphogenetic movements during epiboly by regulating microfilament formation, 12-22, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.