FIGURE

Fig. 3

ID

ZDB-FIG-080129-3

Publication

Woltering et al., 2008 - MiR-10 Represses HoxB1a and HoxB3a in Zebrafish

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Fig. 3

Morpholino knockdown of miR-10 A) ClustalW alignment of the 5 Zebrafish miR-10 precursor sequences. Indicated are the positions of the mature microRNA, the hairloop and the miR-10* (antisense pairing sequence in the hairpin). The target sequences for both morpholino reagent 1 and 2 are indicated with yellow bars (MO1 and MO2). B) Northern blot for all 4 different miR-10 isoforms in morpholino injected embryos at 24, 48 and 72 hpf. There is an absence or very strong downregulation of the mature microRNA in the morpholino injected samples. C) LNA in situ hybridization for miR-10b and miR-10c in 72 hpf miR-10 morphants. The endogenous expression of miR-10b and miR-10c is no longer detected.

Expression Data

Genes:	mir10a mir10b-1 mir10c mir10d
Fish:	WT WT + MO1-mir10 + MO2-mir10 WT + MO3-mir10
Knockdown Reagents:	MO1-mir10 MO2-mir10 MO3-mir10
Anatomical Terms:	hindbrain spinal cord whole organism
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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