Genes required for hyaloid and retinal vasculature development. A-E: Fluorescent images of fli1-EGFP hyaloid vessels on dissected lenses. A: Characteristic pattern of hyaloid vasculature attached to the lens of 5dpf wild type fish. B: Stagnated growth of hyaloid vasculature with less and thicker branches in MAGP1 morphants, and aggregation of vascular endothelial cells at the posterior lens. C: Aberrant oversized branches and lack of patterning in hyaloid vasculature of 5 dpf HS6ST2 morphants. D: hyaloid vessels in 5 dpf mab21l2 morphants are thicker and poorly patterned. E: Poor patterning and abnormal branching of the vessels attached to the lens of 5 dpf obd mutants F: Wholemount retina showing the retinal vasculature of an adult obd mutant exhibiting atypical loops (small arrows) and increased number of vessels. G-P: Alkaline phosphatase staining of hyaloid vasculature on lenses from mutant (left) and wild type larvae (right). G-H: Lens from mgf mutants is reduced in size and shows only residual unorganized vascular tissue at 4 dpf and no vessels at 5 dpf. I: Lens from fe mutants is slightly smaller and has no hyaloid vessels at 3 dpf. J-L: Lens from dsl mutants are much smaller but the hyaloid vasculature displays no apparent abnormalities at 5–8 dpf. M-O: Hyaloid vessels of plt mutants are loose at the back of the lens (asterisks) and the vascular pattern gradually becomes less intricate. P: Vasculature attached to lens of 4–6 dpf lop mutants is similar to vessels on wild type lens. Arrows point from posterior to anterior lens in all panels expect in 4F, where it points from dorsal to ventral retina. Scale bars: 50 μm in all panels except F, 500 μm.
|
