AML1-ETO reprograms hematopoietic cell fate, converting erythropoiesis to granulopoiesis. (A) In situ hybridization of gata1, fluorescent images of zpu.1-EGFP transgenic fish, and in situ hybridization of mpo and l-plastin. AML1-ETO expression results in gata1 downregulation. Subsequently, pu.1 expression is increased. Finally, the accumulated blood cells express the granulocytic cell marker mpo but not the monocytic cell marker l-plastin. All embryos were subjected to the heat treatment and then were collected at designated stages as indicated. (B) Proposed effects of AML1-ETO in hematopoietic progenitor cells. MPC, myeloerythoid progenitor cell; GMP, granulocyte/monocyte progenitor; MEP, megakaryocyte/erythroid progenitor. The red crosses indicate the steps suppressed by AML1-ETO. The parentheses indicate the markers for each cell type or the genes involved in the processes. These data suggest that AML1-ETO reprograms hematopoietic cell fate, resulting in an enrichment of myeloblasts that express mpo (boxed and shaded).
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