FIGURE

Fig. 6

ID

ZDB-FIG-071219-31

Publication

Gribble et al., 2007 - Regulation and function of Dbx genes in the zebrafish spinal cord

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

dbx1a and dbx1b morpholinos cause spinal cord patterning defects. A,B: RT-PCR for dbx1a (A) and dbx1b (B) in morphants show unspliced products (asterisk in each gel) at a concentration of 7 ng/nl for dbx1a and 2.5 ng/nl for dbx1b. Both morpholinos were injected simultaneously to produce dbx1a/1b loss-of-function embryos. C,D: Whole-mount views of a wild-type (C) and dbx1a/1b morphant (D) embryo at 24 hours postfertilization (hpf) with rostral to the left. dbx1a/1b morphants have a shortened rostral/caudal axis and hindbrain morphology defects. E-H: Spinal cord cross-sections of wild-type and dbx1a/1b morphants at 24 hpf. E,F: The nkx6.2 expression is expanded dorsally in dbx1a/1b morphants compared with wild-type. G,H: The msxc expression is unaffected in dbx1a/1b morphants.

Expression Data

Genes:	msx3 nkx6.2
Fish:	AB AB + MO1-dbx1a + MO1-dbx1b
Knockdown Reagents:	MO1-dbx1a MO1-dbx1b
Anatomical Term:	spinal cord
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-dbx1a + MO1-dbx1b
Knockdown Reagents:	MO1-dbx1a MO1-dbx1b
Observed In:	hindbrain post-vent region trunk
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.