FIGURE

Fig. 3

ID

ZDB-FIG-071016-2

Publication

Azuma et al., 2007 - Ewing sarcoma protein ewsr1 maintains mitotic integrity and proneural cell survival in the zebrafish embryo

Other Figures

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Fig. 3

MO-Induced Knockdown of Zebrafish ewsr1b Results in Morphologic Abnormalities in the CNS Accompanied by Apoptosis. A. (a) uninjected, (b) control morpholino (MO), (c) ewsr1a MO , (d) ewsr1a MO with ewsr1a mRNA , (e) ewsr1b MO, and (f) ewsr1b MO and ewsr1b mRNA co-injected embryo. Both ewsr1a and ewsr1b MO injected embryos show dark cells (c and e) in the brain. The asterisk (*) indicates increased apoptosis and abnormal MHB. Co-injection of MO and mRNA rescued normal brain morphology (d and f). All of the images are lateral views of 24hpf zebrafish embryos. B. No increase of apoptosis in the bud stage (a–d), increased apoptosis in the CNS of ewsr1a and ewsr1b -MO injected embryos at 24hpf (g and h compared to e and f). Lateral views of wild type (a and e), control MO injected embryo (b and f), ewsr1a MO injected embryo (c and j) and ewsr1b MO injected embryo (d and h), after TUNEL staining.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-ewsr1a WT + MO1-ewsr1b
Knockdown Reagents:	MO1-ewsr1a MO1-ewsr1b
Observed In:	brain midbrain hindbrain boundary spinal cord
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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