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Fig. 3

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ZDB-FIG-070821-35

Publication

Niikura et al., 2006 - Zebrafish Numb homologue: Phylogenetic evolution and involvement in regulation of left-right asymmetry

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Fig. 3

Whole-mount in situ analysis of left–right asymmetry markers. Expression of bmp4 (A), nkx2.5 (B) lefty2 (C), no tail (D) and shh (E) in control (gal4) and znumb mRNA-injected embryos. In C, deltaD and NICD injected embryos were also examined. Zebrafish fertilized eggs were injected with the indicated mRNA, and embryos were hybridized with anti-sense probes for bmp4 (A), nkx2.5 (B) and lefty2 (C) at around 32 hpf and for no tail and shh at around 9–10 hpf. Injected mRNAs are indicated in each panel, and 2 representative samples of znumb-mRNA-injected embryos are shown in A, B and C. Panels of A, B and C are dorsal views of head portion, and arrowheads indicate signals. In D and E, dorsal views with animal pole to top are shown. Sense probe hybridization was done for all samples, and no significant signal was observed (data not shown).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 123(5), Niikura, Y., Tabata, Y., Tajima, A., Inoue, I., Arai, K.I., and Watanabe, S., Zebrafish Numb homologue: Phylogenetic evolution and involvement in regulation of left-right asymmetry, 407-414, Copyright (2006) with permission from Elsevier. Full text @ Mech. Dev.