Fig. 4

The green fluorescent protein (GFP) expression in 3-month-old stable transgenic fish of gsnl1. A,B: Merged images of whole-mount GFP fluorescence and brightfield microscopy. A: Lateral view. The white lines indicate the positions of sections C-H. B: Dorsal view. The GFP expression was detected in the anterior segment of the eye. C-H: Laser-scanning confocal microscopic images of the transverse sections at the iridocorneal angle. Green, GFP. Blue, nuclear staining by 4′,6-diamidine-2-phenylidole-dihydrochloride (DAPI). C,D: Dorsal region. E,F: Ventral region. G,H: Temporal region. The GFP signal was detected in the annular ligament, a specific connective tissue at the iridocorneal angle. Boxes in C,E,G indicate the positions of the high magnification images of D,F,H, respectively. al, annular ligament; cor, cornea; irs, iris; len, lens; ret, retina. Scale bar = 1 mm in A; 50 μm in C,D.