Coexpression of calsenilin and pancreatic hormones. Double fluorescent in situ hybridization in zebrafish embryos. Zebrafish at 24 hours postfertilization (hpf) and 30 hpf were cohybridized with digoxigenin-labeled antisense calsenilin probe in combination with either DNP-labeled antisense insulin, glucagon, or somatostatin2 probes. Calsenilin was revealed with tyramide-Cy3 substrate (in red) while insulin, glucagon, and somatostatin2 were revealed with tyramide-fluorescein isothiocyanate (in green). Images on the right column represent superposition of the respective left and middle confocal panels.
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