ednras are expressed in the pharyngeal arches. Whole-mount RNA in situ hybridizations in wild-type embryos; lateral views with anterior to the left (A-E,G,H,J,K). Transverse cryosections through the arches (F,I,L). (A) At 14 hpf, ednra1 is expressed in cranial NC. (B) At 16 hpf ednra1 marks three streams of cranial NC as well as NC in the trunk (18 hpf, C). (D) By 24 hpf, ednra1 is expressed throughout the entire DV extent of every arch. (E) Expression in NC cells is excluded from the endodermal pouches but is present in cranial ganglia (32 hpf, arrowheads in D,E). (F) Transverse cryosection at 32 hpf showing that ednra1 is expressed in both the NC and arch ectoderm. (G) ednra2 expression in cranial vasculature at 20 hpf (arrowheads in G,H,J). (H) ednra2 expression in NC cells of the arches at 24 hpf. (I) Co-labeling for dlx2a (red) and ednra2 (blue) showing both genes are expressed in NC cells throughout the arch and excluded from the ectoderm at 28 hpf. (J) At 32 hpf, ednra2 is expressed in NC, but like ednra1, is excluded from the pharyngeal endoderm. (K) ednra2 expression in trunk vasculature at 24 hpf. (L) dlx2a is expressed in NC cells throughout the arch but not the ectoderm at 28 hpf.
|
