FIGURE

Fig. 3

ID

ZDB-FIG-070213-6

Publication

Del Giacco et al., 2006 - Differential regulation of the zebrafish orthopedia1 gene during fate determination of diencephalic neurons

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Fig. 3

Developmental RT-PCR analysis of the otp1 transcripts. Ethidium Bromide-stained agarose gel of RT-PCRs performed using the zotpS and zotpRv primers: DNA Ladder (lane 1), oocyte (lanes 2 and 3), 1-2 cell stage (lane 4), 30% epiboly (lanes 5 and 6), 50% epiboly (lane 7), 8 somite stage (lane 8), 15-20 somite stage (lane 9), 24 hpf (lane 10), 48 hpf (lane 11), brain (lane 12), testis (lane 13), and negative control (lane 14) in absence of cDNA. 80% epiboly, 1-2 somites, and 10 somites samples (not shown) resulted positive after the analysis. On the left of the panel is shown the size of the DNA Ladder bands, on the right is indicated the size of the 2 PCR products, 292 bp and 331 bp, corresponding to the short and the long form of the otp1 mRNA, respectively.

Expression Data

Gene:	otpb
Fish:	AB
Anatomical Terms:	brain oocyte testis whole organism
Stage Range:	1-cell to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.