FIGURE

Fig. 5

ID

ZDB-FIG-061205-13

Publication

Sato et al., 2006 - Semaphorin3D regulates invasion of cardiac neural crest cells into the primary heart field

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

sema3D morphants have fewer cardiomyocytes in the primary heart field. Dorsal views cmlc2 expression in control (A, A′) and sema3D morphants (B, B′). (C) Schematic diagram of inside (I) and outside (O) diameters of cmlc2 expression (purple) and (Y) yolk diameter. Graphs indicate mean ± one standard deviation of diameters measured (μm) in sema3D morphants (red circles, n = 18) and controls (blue triangles, n = 13). The primary heart field is significantly smaller in sema3D morphants (*P = 0.0002, **P = 0.0549). (D) An image of dissociated cmlc2-gfp-positive heart at the 26 hpf, after arrival on neural crest. (E) Count of number of cardiomyocytes from dissociated cmlc2::EGFP embryos. y axis is mean number of EGFP-positive cardiomyocytes, bars indicate standard deviation. Control embryos had 184 ± 15 (n = 30) cardiomyocytes. Both aug-MO and sp-MO-injected embryos had significantly fewer GFP-positive cardiomyocytes (aug-MO: 145 ± 21, n = 23, sp-MO: 154 ± 11, n = 11) than control (*P < 0.0001).

Expression Data

Gene:	myl7
Fish:	WT WT + MO2-sema3d
Knockdown Reagent:	MO2-sema3d
Anatomical Term:	heart primordium
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 298(1), Sato, M., Tsai, H.J., and Yost, H.J., Semaphorin3D regulates invasion of cardiac neural crest cells into the primary heart field, 12-21, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.