Fig. 9

The Brg1-MO injected and yng mutant embryos exhibit reduced peripheral neurons and axons. A: One- to two-cell stage embryos were injected with control-MO or Brg1-MO, and at 24 hpf embryos were fixed and immunostained with antibodies to acetylated tubulin (acTUB) (lateral) or HuC/D (dorsal). Original magnification 200×. B: The yng mutant embryos were fixed and immunostained at 5 dpf as above. Lateral views are presented. The acTUB and HuC/D were detected using AlexaFluor 594- and AlexaFluor 488- labeled secondary antibodies, respectively. Image analysis was performed with conventional fluorescent or confocal microscopy. In A, note the reduced primary motor nerves and Rohon Beard sensory neurons in Brg1-MO-injected embryos compared to control (arrowheads). In B, note the reduced DRGs in the trunk region of yng mutant embryos compared to wild type indicated by arrowheads. Original magnification 200×.