FIGURE

Fig. 8

ID

ZDB-FIG-061110-23

Publication

Pineda et al., 2006 - Knockdown of Nav1.6a Na+ channels affects zebrafish motoneuron development

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Fig. 8

Effects of the 1.6MO on ventrally projecting SMN axons are non cell-autonomous. In wild-type embryo mosaics (A,B), the presence of the 1.6MO (B) or ConMO (A) in ventrally projecting SMNs did not produce abnormally projecting axons. The lineage tracer fluorescein-conjugated dextran (green) was co-injected with the MO. SMN axons were revealed by zn8 immunocytochemistry (red). Ventrally projecting axons (arrows) are both zn8⁺ (red) and GFP⁺ (green), indicating that they contain axons of neurons derived from the MO-injected cell. (C,D) In embryos of the Tg(gata2:GFP) line, GFP⁺ (green) axons were abnormal even though they did not contain the lineage tracer rhodamine-conjugated dextran (red). However, other spinal neurons were positive for the lineage tracer (red), indicating that the 1.6MO had non cell-autonomous effects of ventrally projecting SMNs. Each panel contains a projection of six consecutive confocal sections. Scale bar: 40 μm.

Expression Data

Gene:	alcama
Fish:	WT + MO1-scn8aa
Knockdown Reagent:	MO1-scn8aa
Anatomical Term:	secondary motor neuron
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-scn8aa
Knockdown Reagent:	MO1-scn8aa
Observed In:	secondary motor neuron axon
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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