FIGURE

Fig. 5

ID

ZDB-FIG-060901-5

Publication

Li et al., 2006 - The transcription factor ZBP-89 controls generation of the hematopoietic lineage in zebrafish and mouse embryonic stem cells

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Fig. 5

Effect of forced expression of ZBP-89 on hematopoietic and vascular development in zebrafish embryos. (A-J) Wild type (A,C,E,G,I) and ZBP-89-overexpressing (B,D,F,H,J) 18 hpf (A-F) or 24 hpf (G-J) zebrafish embryos. In situ hybridization of wild-type embryos overexpressing ZBP-89 reveals a marked increase in the expression of scl (B), lmo2 (D) and gata1 (F), but a marked reduction in intersomitic expression of the endothelial markers flk1 (H) and tie1 (J), when compared with the respective untreated wild-type embryos (A,C,E,G,I), (K,L) Wild-type (K) and ZBP-89-overexpressing (L) Tg (flk1:EGFP) zebrafish embryos examined at 24 hpf. Overexpression of ZBP-89 caused a significant reduction in flk1 expression in the axial (arrows), brain (short arrow) and intersomitic (arrowheads) blood vessels when compared with the control embryo.

Expression Data

Genes:	gata1a kdrl lmo2 tal1 tie1
Fish:	WT
Anatomical Terms:	anterior lateral mesoderm intermediate cell mass of mesoderm intersegmental vessel
Stage Range:	14-19 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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