FIGURE

Fig. 3

ID

ZDB-FIG-060706-15

Publication

Gibert et al., 2006 - Induction and prepatterning of the zebrafish pectoral fin bud requires axial retinoic acid signaling

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Fig. 3

Retinoic acid rescues pectoral fin field induction in the absence of paraxial mesoderm. Whole-mount in situ hybridizations. Anterior is towards the left, dorsal views. (A-D) Expression of tbx5 in pectoral fin buds is unchanged in ntl(B), but reduced in spt (C) and absent in ntl/spt double-mutant embryos (D). (E-M) Application of 10^-8 M RA, beginning at 10 hpf, rescues tbx5 expression in ntl/spt pectoral fins (arrowheads in I,K); rescue occurs in the absence of myogenic differentiation, demonstrated by the absence of myod (J,K) and α-tropomyosin-1 (tpm1) expression (L,M) in RA-treated double mutants. Four rescue experiments, each with 40 embryos, were performed (total numbers of phenotypes: 32 spt, 35 ntl, 11 ntl/spt, 82 siblings without mutant phenotype).

Expression Data

Genes:	myod1 tbx5a tpm1
Fish:	WT tbx16^b104/b104 (AB) tbxta^tc41/tc41 tbx16^b104/b104; tbxta^tc41/tc41
Conditions:	constant dark chemical treatment: all-trans-retinoic acid, constant dark
Anatomical Terms:	mesoderm pectoral fin bud somite
Stage Range:	Prim-5 to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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