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Manifestation of defects in the presumptive cloaca of transgenic embryos. (A-H) gata3 expression in the kidney terminus of wild-type (A-D) and transgenic (E-H) sibling embryos at the 22-28 somite (som) stages. Note the lack of remodeling in the transgenic embryos compared with wild types (the wild-type cloacal opening is obscured in these images as a result of the fixation and subsequent processing). (I-L) Wild-type and transgenic sibling embryos heat shocked at 80% epiboly were stained with BODIPY TR methyl ester and imaged with a confocal microscope. Pseudocoloring was used to highlight the kidney terminus in red and the proctodeum and epidermis in blue. (I,K) The kidney terminus in 22-somite wild-type embryos (I) has reached the ventral limit of the tail, while it has not in transgenic siblings (K). (J,L) At 25 somites, the kidney terminus opens to the exterior in some wild type fish (J), while the kidney has swelled up in transgenic siblings (L).
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