Fig. 1
Pharyngula (24 hr) wild-type (WT, A) and mutant embryos (B–G, the gene name abbreviations on this and other figures indicate homozygous mutants for the loci indicated). The head of the cyc;flh double mutant (E) is similar to cyc- (B; see Hatta et al., 1991), whereas the body is similar to flh- (C), with respect to both midline somite fusion and lack of ventral body curvature. The tail of the flh;ntl double mutant (F) is like ntl- (D), and in this example there is a patch of fused somites, as present in flh-, just dorsal to the yolk extension. Somite fusion is variable, occurring in 43% (15/35) of embryos subsequently confirmed to be flh;ntl double mutants by PCR genotyping (see Materials and Methods). The cyc;ntl double mutant (G) combines the cyc- head and ntl- tail phenotypes. Scale bar, 250 um. |
Reprinted from Developmental Biology, 187(2), Halpern, M.E., Hatta, K., Amacher, S.L., Talbot, W.S., Yan, Y.-L., Thisse, B., Thisse, C., Postlethwait, J.H., and Kimmel, C.B., Genetic interactions in zebrafish midline development, 154-170, Copyright (1997) with permission from Elsevier. Full text @ Dev. Biol.