Expression of sna-1 in wild-type, spt and ntl mutant postgastrula embryos, investigated by whole-mount in situ hybridisation (A,B,C,D,E,G,H,J,L,M) and anti-Sna-1 antibody staining (E, small photo, F,K). (A,B) 95% epiboly, dorsal view: (A) wild-type, (B) ntl. (C,D) 4-somite stage, optical cross section at level of presomitic mesoderm: (C) wild-type, (D) ntl. (E, small photo) 5-somite stage, dorsal view on somites. (F) 15-somite stage, spread wild-type embryo, dorsal view on posterior part of the embryo up to somite 12. (E, big photo, G,H) 8-somite stage, dorsal view, spread embryo: (E) wild type; (G) ntl ; (H) spt. (K) 15-somite stage, wild type, lateral view on somites 10-13. (J,L,M) 22 hour embryos, lateral view of the tail and the posterior part of the trunk; borders of embryo outlined by dots: (J) wildtype; (L) ntl ; (M) spt. Abbreviations: muscle pioneer precursor (mpp), notochord (n), somite (s), tailbud (tb). For details see text.
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