Fig. 5

Expression of zebrafish CRMP2 at 30 hpf and 48 hpf, in comparison with PlexinA4. At 30 hpf, CRMP2 is expressed in the drc, vrc, vcc, TG, RB cells, and the hindbrain (A, A′, B). CRMP2 expression overlaps with PlexinA4 in all of these structures with the exception of RB cells, where Plexin A4 is very weakly expressed. Plexin A4 is also expressed in the dorsal mesencephalon (arrowhead), and dorsal diencephalon (thick arrow) (C, D). By 48 hpf, CRMP2 (E, E′, F) and PlexinA4 (G, G′, H) have a dramatic increase in the amount of expression in the central nervous system. CRMP2 expression becomes more diffuse throughout the entire brain, although it is still localized in some regions of the brain (Fig. 5E, E′, F, sections 5I, 5J, 5K). At 48 hpf, CRMP2 is clearly detected in the developing retinal ganglion layer (E, F and section I). At 48 hpf, CRMP2 is also expressed in the otic vesicle (E, E′), as evident in cross-sections (K). PlexinA4 is also clearly expressed in the otic vesicle (G, G′). Figures B, D, F and H are de-yolked, dorsal views. A′, C′ E′ and G′ are enlargements of staining from just behind the eye of A, C, E and G respectively. Figures I, J, and K are 7 μm sections taken through the regions of the brain as outlined in E.