FIGURE

Fig. 2

ID

ZDB-FIG-050721-7

Publication

Qian et al., 2005 - Microarray analysis of zebrafish cloche mutant using amplified cDNA and identification of potential downstream target genes

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Fig. 2

Analysis of amplified cDNA quality. A: DNA electrophoresis analysis. A total of 100 ng of each amplified cDNAs derived from the single 18-somite clo homozygous mutant (lanes 2, 4, and 6) and wild-type or heterozygous sibling embryos (lanes 1, 3 and 5) was separated on 1% agarose gel. Ethidium bromide staining shows an evenly distributed smear of DNA in each lane. B: Virtual Northern analysis. cDNAs of A were transferred to a Hybone-N+ membrane and probed with digoxigenin-labeled flk1, gata1, e1-globin and actin. Whereas elf1a transcript was maintained at a similar level in all the samples, expression of flk1, gata1, and e1-globin were either absent or drastically reduced in the clo mutant embryos (lanes 2, 4, and 6).

Expression Data

Genes:	efna2a gata1a hbbe1.1 kdrl
Fish:	WT npas4l^s5
Anatomical Term:	unspecified
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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