Fig. 2

Isolation of mutants with disrupted migration of the nVII motor neurons. Morphology and Isl1-GFP expression in the wild-type (A,E,I), llk^rw16 (B,F,J), ord ^rw71 (C,G,K), and tri ^rw75 homozygous embryos (D,H,L) at 2 dpf (A-H) and 30 hpf (I-L). In the wild-type embryos, the nVII motor neurons are located in r6 (E,I, arrows). In contrast, in the llk ^rw16 and tri ^rw75 embryos the nVII motor neurons are located in r4 (F,J,H,L, arrows). In the ord ^rw71 embryos, the nVII motor neurons are located in r4 and r5 (G,K, arrowheads indicate cells migrating into r5). The cells that migrated into r5 became detached from the surface of hindbrain, and were scattered inside the hindbrain. The tri ^rw75 embryos show severe defects in the extension of the trunk region (D). The llk and ord embryos did not show any morphological abnormalities, in contrast to the tri embryos (B,C). (A-D, I-L) Lateral views; anterior is to the left, (E-H) dorsal views; anterior is to the top. Scale bars: 50 µm.