FIGURE

Fig. 5

ID

ZDB-FIG-050307-5

Publication

Amoyel et al., 2005 - Wnt1 regulates neurogenesis and mediates lateral inhibition of boundary cell specification in the zebrafish hindbrain

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Fig. 5

Neurogenesis at 48 hours in wnt MO-injected embryos. (A,B) Reticulospinal neurons labelled by RMO-44 antibody (arrows) are still present (11/14 embryos), and cranial motor nerves (V, VI, VII, IX and X) labelled with tbx20 (C,D) are mildly hypomorphic (11/11 embryos). The number of dbx1a-expressing cells appears to be similar in uninjected and morphant embryos (E,F), but their distribution in stripes adjacent to boundaries is disrupted (20/20 embryos). Sections of uninjected (I,J) and wnt1MO embryos (K,L) show that in uninjected embryos there are many neurons expressing dbx1a in the mantle zone (MZ); in the morphants, most dbx1a expression occurs in the ventricular zone (VZ) of progenitors (4/4 embryos), as determined by DAPI stain (J,L) and by absence of Hu staining (not shown). (G,H) gfap expression occurs in stripes adjacent to boundaries in uninjected embryos (arrows in G), and ectopically in wnt1 MO embryos (H, 12/13 embryos), suggesting that neural progenitors have switched fate to glial cells. Scale bar: in A, 50 µm for A,B,I-L; in C, 100 µm for C-H.

Expression Data

Genes:	dbx1a gfap tbx20
Fish:	WT WT + MO1-wnt1
Knockdown Reagent:	MO1-wnt1
Anatomical Terms:	cranial ganglion hindbrain
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-wnt1
Knockdown Reagent:	MO1-wnt1
Observed In:	cranial nerve IX cranial nerve VI cranial nerve VII cranial nerve X glial cell motor neuron neurogenesis
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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