FIGURE

Fig. 6

ID

ZDB-FIG-050214-5

Publication

Dooley et al., 2005 - Zebrafish scl functions independently in hematopoietic and endothelial development

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Fig. 6

Analysis of early gene expression in the scl morphants. Expression of gata2 (A, B), lmo2 (C, D), dra (E, F) and fli1b (I, J) at the 10- to 12-somite stage in scl morphants and uninjected controls. As an internal control, the gata2 ISH was performed as a double with myoD, a marker of the somitic tissue, detected in red (A, B). (I, J) Double ISH using probes for lmo2 (red arrow) and fli1b (green arrow) in uninjected and scl morphant embryo, showing higher magnification of posterior mesoderm. All embryos were flat mounted and shown with anterior to the left. In D, a schematic illustrates the orientation of embryos and regions of erythroid progenitors and angioblasts.

Expression Data

Genes:	drl gata2a lmo2 myod1
Fish:	WT WT + MO1-tal1 + MO2-tal1
Knockdown Reagents:	MO1-tal1 MO2-tal1
Anatomical Terms:	lateral mesoderm somite
Stage:	10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 277(2), Dooley, K.A., Davidson, A.J., and Zon, L.I., Zebrafish scl functions independently in hematopoietic and endothelial development, 522-536, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.