CRISPR

CRISPR2-dhcr7

ID

ZDB-CRISPR-250312-2

Name

CRISPR2-dhcr7

Previous Names

None

Target

dhcr7 (1)

Sequence

5' - ATCCAGTAGAGGGTGACTTA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "TGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
oi14	dhcr7

Expression

Gene expression in Wild Types + CRISPR2-dhcr7

No data available

Phenotype

Phenotype resulting from CRISPR2-dhcr7

No data available

Phenotype of all Fish created by or utilizing CRISPR2-dhcr7

Phenotype	Fish	Conditions	Figures
telencephalon lysosome increased amount, abnormal	dhcr7^oi14/oi14	standard conditions	Fig. 3 from Miyazaki et al., 2024
brain cholesterol decreased amount, abnormal	dhcr7^oi14/oi14	standard conditions	Fig. 1 from Miyazaki et al., 2024
brain decreased area, abnormal	dhcr7^oi14/oi14	standard conditions	Fig. 1 from Miyazaki et al., 2024
brain serotonin increased amount, abnormal	dhcr7^oi14/oi14	standard conditions	Fig. 4 from Miyazaki et al., 2024
optic tectum decreased area, abnormal	dhcr7^oi14/oi14	standard conditions	Fig. 1 from Miyazaki et al., 2024
cerebellum decreased area, abnormal	dhcr7^oi14/oi14	standard conditions	Fig. 1 from Miyazaki et al., 2024
aggressive behavior decreased process quality, abnormal	dhcr7^oi14/oi14	standard conditions	Fig. 4 from Miyazaki et al., 2024
optic tectum myelination decreased process quality, abnormal	dhcr7^oi14/oi14	standard conditions	Fig. 3 from Miyazaki et al., 2024
whole organism decreased length, abnormal	dhcr7^oi14/oi14	standard conditions	Fig. 1 from Miyazaki et al., 2024
brain ab4-sqstm1 labeling increased amount, abnormal	dhcr7^oi14/oi14	standard conditions	Fig. 3 from Miyazaki et al., 2024
telencephalon myelination decreased process quality, abnormal	dhcr7^oi14/oi14	standard conditions	Fig. 3 from Miyazaki et al., 2024
brain dhcr7 expression decreased amount, abnormal	dhcr7^oi14/oi14	standard conditions	Fig. 1 from Miyazaki et al., 2024
telencephalon synaptic cleft blurry, abnormal	dhcr7^oi14/oi14	standard conditions	Fig. 4 from Miyazaki et al., 2024
brain cholesta-5,7-dien-3beta-ol increased amount, abnormal	dhcr7^oi14/oi14	standard conditions	Fig. 1 from Miyazaki et al., 2024
brain ab1-map1lc3b labeling decreased amount, abnormal	dhcr7^oi14/oi14	standard conditions	Fig. 3 from Miyazaki et al., 2024
brain noradrenaline increased amount, abnormal	dhcr7^oi14/oi14	standard conditions	Fig. 4 from Miyazaki et al., 2024
behavioral fear response decreased process quality, abnormal	dhcr7^oi14/oi14	standard conditions	Fig. 4 from Miyazaki et al., 2024
telencephalon decreased area, abnormal	dhcr7^oi14/oi14	standard conditions	Fig. 1 from Miyazaki et al., 2024
brain dopamine increased amount, abnormal	dhcr7^oi14/oi14	standard conditions	Fig. 4 from Miyazaki et al., 2024
optic tectum Wnt signaling pathway decreased process quality, abnormal	dhcr7^oi14/oi14; kyu2Tg/kyu2Tg	standard conditions	Fig. S2 from Miyazaki et al., 2024
optic tectum posterior side d2EGFP expression decreased amount, abnormal	dhcr7^oi14/oi14; kyu2Tg/kyu2Tg	standard conditions	Fig. S2 from Miyazaki et al., 2024
optic tectum glioblast Tomato expression decreased amount, abnormal	dhcr7^oi14/oi14; nns17Tg	standard conditions	Fig. 2 from Miyazaki et al., 2024
optic tectum neuronal stem cell Tomato expression decreased amount, abnormal	dhcr7^oi14/oi14; nns17Tg	standard conditions	Fig. 2 from Miyazaki et al., 2024
optic tectum neuronal stem cell disorganized, abnormal	dhcr7^oi14/oi14; nns17Tg	standard conditions	Fig. 2 from Miyazaki et al., 2024
optic tectum neuron differentiation decreased process quality, abnormal	dhcr7^oi14/oi14; nns17Tg	standard conditions	Fig. 2 from Miyazaki et al., 2024
cerebellum neuron Kaede expression decreased amount, abnormal	dhcr7^oi14/oi14; rw0130aTg	standard conditions	Fig. 2 from Miyazaki et al., 2024
telencephalon neuron Kaede expression decreased amount, abnormal	dhcr7^oi14/oi14; rw0130aTg	standard conditions	Fig. 2 from Miyazaki et al., 2024
cerebellum neuron decreased amount, abnormal	dhcr7^oi14/oi14; rw0130aTg	standard conditions	Fig. 2 from Miyazaki et al., 2024
optic tectum neuron Kaede expression decreased amount, abnormal	dhcr7^oi14/oi14; rw0130aTg	standard conditions	Fig. 2 from Miyazaki et al., 2024
telencephalon neuron decreased amount, abnormal	dhcr7^oi14/oi14; rw0130aTg	standard conditions	Fig. 2 from Miyazaki et al., 2024
optic tectum neuron decreased amount, abnormal	dhcr7^oi14/oi14; rw0130aTg	standard conditions	Fig. 2 from Miyazaki et al., 2024

Citations