CRISPR

CRISPR2-rptor

ID

ZDB-CRISPR-240808-2

Name

CRISPR2-rptor

Previous Names

None

Target

rptor (1)

Sequence

5' - GGCAGAGGCATCTGAGCTAG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
au93	rptor

Expression

Gene expression in Wild Types + CRISPR2-rptor

No data available

Phenotype

Phenotype resulting from CRISPR2-rptor

No data available

Phenotype of all Fish created by or utilizing CRISPR2-rptor

Phenotype	Fish	Conditions	Figures
ceratohyal cartilage decreased length, abnormal	rptor^au93/au93 (AB)	chemical treatment by environment: chloroquine	Fig. 10 from Tucker et al., 2024
ethmoid cartilage length, ameliorated	rptor^au93/au93 (AB)	chemical treatment by environment: chloroquine	Fig. 10 from Tucker et al., 2024
neurocranium decreased length, abnormal	rptor^au93/au93 (AB)	control	Fig. 4 , Fig. 10 , Fig. 11 from Tucker et al., 2024
ethmoid cartilage decreased length, abnormal	rptor^au93/au93 (AB)	control	Fig. 4 , Fig. 10 , Fig. 11 from Tucker et al., 2024
Meckel's cartilage length, ameliorated	rptor^au93/au93 (AB)	chemical treatment by environment: chloroquine	Fig. 10 from Tucker et al., 2024
trabecula cranii chondrocyte decreased area, abnormal	rptor^au93/au93 (AB)	standard conditions	Fig. 5 from Tucker et al., 2024
eye decreased width, abnormal	rptor^au93/au93 (AB)	control	Fig. 4 , Fig. 10 , Fig. 11 from Tucker et al., 2024
ethmoid cartilage decreased width, abnormal	rptor^au93/au93 (AB)	chemical treatment by environment: chloroquine	Fig. 10 from Tucker et al., 2024
trabecula cranii decreased length, abnormal	rptor^au93/au93 (AB)	control	Fig. 4 , Fig. 10 , Fig. 11 from Tucker et al., 2024
autophagy increased occurrence, abnormal	rptor^au93/au93 (AB)	standard conditions	Fig. 9 from Tucker et al., 2024
trabecula cranii chondrocyte decreased amount, abnormal	rptor^au93/au93 (AB)	standard conditions	Fig. 5 from Tucker et al., 2024
eye decreased width, abnormal	rptor^au93/au93 (AB)	chemical treatment by environment: chloroquine	Fig. 10 from Tucker et al., 2024
neurocranium length, ameliorated	rptor^au93/au93 (AB)	chemical treatment by environment: chloroquine	Fig. 10 from Tucker et al., 2024
Meckel's cartilage decreased length, abnormal	rptor^au93/au93 (AB)	control	Fig. 4 , Fig. 7 , Fig. 10 , Fig. 11 from Tucker et al., 2024
whole organism rptor expression decreased amount, abnormal	rptor^au93/au93 (AB)	standard conditions	Fig. 2 from Tucker et al., 2024
ceratohyal cartilage decreased length, abnormal	rptor^au93/au93 (AB)	control	Fig. 4 , Fig. 10 , Fig. 11 from Tucker et al., 2024
whole organism viability, abnormal	rptor^au93/au93 (AB)	chemical treatment by environment: chloroquine	text only from Tucker et al., 2024
ethmoid cartilage decreased width, abnormal	rptor^au93/au93 (AB)	control	Fig. 4 , Fig. 10 , Fig. 11 from Tucker et al., 2024
Meckel's cartilage length, ameliorated	rptor^au93/au93 (AB)	chemical treatment by environment: EC 3.4.22.56 (caspase-3) inhibitor	Fig. 7 from Tucker et al., 2024
whole organism Ab6-map1lc3b labeling increased amount, abnormal	rptor^au93/au93 (AB)	standard conditions	Fig. 9 from Tucker et al., 2024
ceratobranchial cartilage decreased amount, abnormal	rptor^au93/au93 (AB)	standard conditions	Fig. 4 from Tucker et al., 2024
cranial neural crest cell death increased occurrence, abnormal	rptor^au93/au93; y1Tg (AB)	standard conditions	Fig. 6 from Tucker et al., 2024
cranial neural crest cell death occurrence, ameliorated	rptor^au93/au93; y1Tg (AB)	chemical treatment by environment: chloroquine	Fig. 12 from Tucker et al., 2024
Meckel's cartilage length, ameliorated	atg7^{sa14768/sa14768}; rptor^au93/au93 (AB)	standard conditions	Fig. 11 from Tucker et al., 2024
trabecula cranii length, ameliorated	atg7^{sa14768/sa14768}; rptor^au93/au93 (AB)	standard conditions	Fig. 11 from Tucker et al., 2024
ceratohyal cartilage decreased length, abnormal	atg7^{sa14768/sa14768}; rptor^au93/au93 (AB)	standard conditions	Fig. 11 from Tucker et al., 2024
ethmoid cartilage width, ameliorated	atg7^{sa14768/sa14768}; rptor^au93/au93 (AB)	standard conditions	Fig. 11 from Tucker et al., 2024
eye width, ameliorated	atg7^{sa14768/sa14768}; rptor^au93/au93 (AB)	standard conditions	Fig. 11 from Tucker et al., 2024
ethmoid cartilage length, ameliorated	atg7^{sa14768/sa14768}; rptor^au93/au93 (AB)	standard conditions	Fig. 11 from Tucker et al., 2024
pharyngeal arch autophagy increased occurrence, abnormal	rptor^au93/au93; zf155Tg (AB)	standard conditions	Fig. 8 from Tucker et al., 2024

Citations