CRISPR

CRISPR1-pikfyve

ID

ZDB-CRISPR-240626-2

Name

CRISPR1-pikfyve

Previous Names

None

Target

Sequence

5' - GCTAGTTGTGGGGATCAT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "AGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
shz1	pikfyve

Expression

Gene expression in Wild Types + CRISPR1-pikfyve

No data available

Phenotype

Phenotype resulting from CRISPR1-pikfyve

No data available

Phenotype of all Fish created by or utilizing CRISPR1-pikfyve

Phenotype	Fish	Conditions	Figures
lens opaque, abnormal	pikfyve^shz1/shz1 (AB)	standard conditions	Figure 3 from Mei et al., 2022
lens vacuole increased amount, abnormal	pikfyve^shz1/shz1 (AB)	standard conditions	Figure 3 , Figure 4. , Figure 6 from Mei et al., 2022
whole organism dead, abnormal	pikfyve^shz1/shz1 (AB)	standard conditions	Figure 3 from Mei et al., 2022
lens vacuole amount, ameliorated	pikfyve^shz1/shz1 (AB)	chemical treatment by environment: Bafilomycin A1(Baf-A1)	Figure 6 from Mei et al., 2022
lens fiber cell disorganized, abnormal	pikfyve^shz1/shz1 (AB)	standard conditions	Figure 4. from Mei et al., 2022
lens vacuole increased amount, abnormal	pikfyve^shz1/+ (AB)	standard conditions	Figure 3 from Mei et al., 2022
lens vacuole amount, ameliorated	pikfyve^shz1/shz1; shz2Tg (AB)	standard conditions	Figure 3 from Mei et al., 2022
lens vacuole amount, ameliorated	pikfyve^shz1/shz1; shz3Tg (AB)	standard conditions	Figure 3 from Mei et al., 2022

Citations