CRISPR

CRISPR1-tspearb

ID

ZDB-CRISPR-240509-25

Name

CRISPR1-tspearb

Previous Names

None

Target

tspearb (1)

Sequence

5' - GGTGCAGGATGGTATACGCA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
omf107	tspearb

Expression

Gene expression in Wild Types + CRISPR1-tspearb

No data available

Phenotype

Phenotype resulting from CRISPR1-tspearb

No data available

Phenotype of all Fish created by or utilizing CRISPR1-tspearb

Phenotype	Fish	Conditions	Figures
anal fin lepidotrichium decreased branchiness, abnormal	tspearb^{omf107/omf107}; tspeara^{omf106/omf106}	standard conditions	Fig. S14 from Jackson et al., 2023
pelvic fin lepidotrichium decreased branchiness, abnormal	tspearb^{omf107/omf107}; tspeara^{omf106/omf106}	standard conditions	Fig. S14 from Jackson et al., 2023
whole organism fgf1b expression decreased amount, abnormal	tspearb^{omf107/omf107}; tspeara^{omf106/omf106}	standard conditions	Fig. S16, Fig. S17 from Jackson et al., 2023
dorsal fin lepidotrichium decreased branchiness, abnormal	tspearb^{omf107/omf107}; tspeara^{omf106/omf106}	standard conditions	Fig. S14 from Jackson et al., 2023
ceratobranchial 5 tooth disorganized, abnormal	tspearb^{omf107/omf107}; tspeara^{omf106/omf106}	standard conditions	Fig. 5 from Jackson et al., 2023
caudal fin fin regeneration decreased process quality, abnormal	tspearb^{omf107/omf107}; tspeara^{omf106/omf106}	amputation: caudal fin	Fig. 5 from Jackson et al., 2023
ceratobranchial 5 tooth decreased thickness, abnormal	tspearb^{omf107/omf107}; tspeara^{omf106/omf106}	standard conditions	Fig. 5 from Jackson et al., 2023
whole organism sox8b expression decreased amount, abnormal	tspearb^{omf107/omf107}; tspeara^{omf106/omf106}	standard conditions	Fig. S16 from Jackson et al., 2023
whole organism ambn expression decreased amount, abnormal	tspearb^{omf107/omf107}; tspeara^{omf106/omf106}	standard conditions	Fig. S17 from Jackson et al., 2023
precaudal vertebra increased width, abnormal	tspearb^{omf107/omf107}; tspeara^{omf106/omf106}	standard conditions	Fig. S14 from Jackson et al., 2023
whole organism mmp20b expression decreased amount, abnormal	tspearb^{omf107/omf107}; tspeara^{omf106/omf106}	standard conditions	Fig. S17 from Jackson et al., 2023
whole organism kcnk5a expression decreased amount, abnormal	tspearb^{omf107/omf107}; tspeara^{omf106/omf106}	standard conditions	Fig. S16 from Jackson et al., 2023
whole organism mustn1a expression decreased amount, abnormal	tspearb^{omf107/omf107}; tspeara^{omf106/omf106}	standard conditions	Fig. S16 from Jackson et al., 2023
precaudal vertebra bifurcated, abnormal	tspearb^{omf107/omf107}; tspeara^{omf106/omf106}	standard conditions	Fig. S14 from Jackson et al., 2023
whole organism mmp20a expression decreased amount, abnormal	tspearb^{omf107/omf107}; tspeara^{omf106/omf106}	standard conditions	Fig. S17 from Jackson et al., 2023
whole organism scpp5 expression decreased amount, abnormal	tspearb^{omf107/omf107}; tspeara^{omf106/omf106}	standard conditions	Fig. S16, Fig. S17 from Jackson et al., 2023
ceratobranchial 5 tooth decreased amount, abnormal	tspearb^{omf107/omf107}; tspeara^{omf106/omf106}	standard conditions	Fig. 5 from Jackson et al., 2023
ceratobranchial 5 tooth bone mineralization decreased process quality, abnormal	tspearb^{omf107/omf107}; tspeara^{omf106/omf106}	standard conditions	Fig. 5 from Jackson et al., 2023
whole organism scpp7 expression decreased amount, abnormal	tspearb^{omf107/omf107}; tspeara^{omf106/omf106}	standard conditions	Fig. S16 from Jackson et al., 2023
whole organism odam expression decreased amount, abnormal	tspearb^{omf107/omf107}; tspeara^{omf106/omf106}	standard conditions	Fig. S17 from Jackson et al., 2023
whole organism cdkn1a expression increased amount, abnormal	tspearb^{omf107/omf107}; tspeara^{omf106/omf106}	standard conditions	Fig. S16 from Jackson et al., 2023
whole organism dlx2b expression increased amount, abnormal	tspearb^{omf107/omf107}; tspeara^{omf106/omf106}	standard conditions	Fig. S16 from Jackson et al., 2023
whole organism enam expression decreased amount, abnormal	tspearb^{omf107/omf107}; tspeara^{omf106/omf106}	standard conditions	Fig. S16, Fig. S17 from Jackson et al., 2023

Citations