CRISPR

CRISPR2-il11ra

ID

ZDB-CRISPR-240123-1

Name

CRISPR2-il11ra

Previous Names

None

Target

il11ra (1)

Sequence

5' - ATGGTGGAGTTAGATCCCAG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "CGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
bns251	il11ra

Expression

Gene expression in Wild Types + CRISPR2-il11ra

No data available

Phenotype

Phenotype resulting from CRISPR2-il11ra

No data available

Phenotype of all Fish created by or utilizing CRISPR2-il11ra

Phenotype	Fish	Conditions	Figures
cardiac ventricle cilp2 expression increased amount, abnormal	il11ra^{bns251/bns251}	ablation: heart	Fig. 5. from Allanki et al., 2021
cardiac ventricle tgfb2 expression increased amount, abnormal	il11ra^{bns251/bns251}	ablation: heart	Fig. 5. from Allanki et al., 2021
cardiac ventricle fn1b expression decreased amount, abnormal	il11ra^{bns251/bns251}	ablation: heart	Fig. 3. from Allanki et al., 2021
regenerating fin fgf20a expression decreased amount, abnormal	il11ra^{bns251/bns251}	amputation: caudal fin	Fig. 3. from Allanki et al., 2021
cardiac ventricle il11ra expression decreased amount, abnormal	il11ra^{bns251/bns251}	ablation: heart	Fig. 3. from Allanki et al., 2021
ventral fin fold fin regeneration decreased process quality, abnormal	il11ra^{bns251/bns251}	amputation: ventral fin fold	Fig. 2. from Allanki et al., 2021
cardiac ventricle acta2 expression increased amount, abnormal	il11ra^{bns251/bns251}	ablation: heart	Fig. 5. from Allanki et al., 2021
regenerating tissue cardiac muscle cell proliferation decreased occurrence, abnormal	il11ra^{bns251/bns251}	ablation: heart	Fig. 4. from Allanki et al., 2021
cardiac ventricle fgf20a expression decreased amount, abnormal	il11ra^{bns251/bns251}	ablation: heart	Fig. 3. from Allanki et al., 2021
cardiac ventricle mylka expression increased amount, abnormal	il11ra^{bns251/bns251}	ablation: heart	Fig. 5. from Allanki et al., 2021
regenerating tissue retinol metabolic process decreased process quality, abnormal	il11ra^{bns251/bns251}	ablation: heart	Fig. 3. from Allanki et al., 2021
cardiac muscle cell substrate-dependent cell migration, cell extension decreased process quality, abnormal	il11ra^{bns251/bns251}	ablation: heart	Fig. 4. from Allanki et al., 2021
cardiac ventricle egr2b expression increased amount, abnormal	il11ra^{bns251/bns251}	ablation: heart	Fig. 5. from Allanki et al., 2021
cardiac ventricle loxa expression increased amount, abnormal	il11ra^{bns251/bns251}	ablation: heart	Fig. 5. from Allanki et al., 2021
regenerating tissue endocardium ab1-elna labeling increased amount, abnormal	il11ra^{bns251/bns251}	ablation: heart	Fig. 5. from Allanki et al., 2021
cardiac ventricle tgfb1a expression increased amount, abnormal	il11ra^{bns251/bns251}	ablation: heart	Fig. 5. from Allanki et al., 2021
cardiac ventricle regeneration fibroblast Ab7-acta2 labeling increased amount, abnormal	il11ra^{bns251/bns251}	ablation: heart	Fig. 5. from Allanki et al., 2021
cardiac ventricle ab2-fn labeling decreased amount, abnormal	il11ra^{bns251/bns251}	ablation: heart	Fig. 3. from Allanki et al., 2021
cardiac ventricle proliferative region ab2-pcna labeling decreased amount, abnormal	il11ra^{bns251/bns251}	ablation: heart	Fig. 4. from Allanki et al., 2021
cardiac ventricle socs3b expression increased amount, abnormal	il11ra^{bns251/bns251}	ablation: heart	Fig. 5. from Allanki et al., 2021
cardiac ventricle connective tissue replacement involved in inflammatory response wound healing increased occurrence, abnormal	il11ra^{bns251/bns251}	ablation: heart	Fig. 2. from Allanki et al., 2021
cardiac ventricle vcanb expression increased amount, abnormal	il11ra^{bns251/bns251}	ablation: heart	Fig. 5. from Allanki et al., 2021
cardiac ventricle extracellular matrix organization increased occurrence, abnormal	il11ra^{bns251/bns251}	ablation: heart	Fig. 5. from Allanki et al., 2021
cardiac ventricle socs3b expression decreased amount, abnormal	il11ra^{bns251/bns251}	ablation: heart	Fig. 3. from Allanki et al., 2021
cardiac ventricle aldh1a2 expression decreased amount, abnormal	il11ra^{bns251/bns251}	ablation: heart	Fig. 3. from Allanki et al., 2021
cardiac ventricle ab1-aldh1a2 labeling decreased amount, abnormal	il11ra^{bns251/bns251}	ablation: heart	Fig. 3. from Allanki et al., 2021
cardiac ventricle egr1 expression increased amount, abnormal	il11ra^{bns251/bns251}	ablation: heart	Fig. 5. from Allanki et al., 2021
regenerating fin fn1b expression decreased amount, abnormal	il11ra^{bns251/bns251}	amputation: caudal fin	Fig. 3. from Allanki et al., 2021
cardiac ventricle elnb expression increased amount, abnormal	il11ra^{bns251/bns251}	ablation: heart	Fig. 5. from Allanki et al., 2021
cardiac ventricle elastic fiber assembly increased occurrence, abnormal	il11ra^{bns251/bns251}	ablation: heart	Fig. 5. from Allanki et al., 2021
cardiac ventricle transforming growth factor beta receptor signaling pathway increased occurrence, abnormal	il11ra^{bns251/bns251}	ablation: heart	Fig. 5. from Allanki et al., 2021
scale regeneration decreased process quality, abnormal	il11ra^{bns251/bns251}	amputation: scale	Fig. 2. from Allanki et al., 2021
cardiac ventricle regulation of receptor signaling pathway via JAK-STAT decreased occurrence, abnormal	il11ra^{bns251/bns251}	ablation: heart	Fig. 5. from Allanki et al., 2021
caudal fin fin regeneration decreased process quality, abnormal	il11ra^{bns251/bns251}	amputation: caudal fin	Fig. 2. from Allanki et al., 2021
cardiac ventricle regeneration decreased process quality, abnormal	il11ra^{bns251/bns251}; ae1Tg/ae1Tg	ablation: heart	Fig. 4. from Allanki et al., 2021
cardiac ventricle EGFP expression decreased amount, abnormal	il11ra^{bns251/bns251}; ae1Tg/ae1Tg	ablation: heart	Fig. 4. from Allanki et al., 2021
endocardium epithelial to mesenchymal transition increased occurrence, abnormal	il11ra^{bns251/bns251}; cn9Tg/cn9Tg; cz1701Tg/cz1701Tg	ablation: heart, chemical treatment by environment: afimoxifene	Fig. 6. from Allanki et al., 2021
regenerating tissue retinol metabolic process decreased process quality, abnormal	il11ra^{bns251/bns251}; hu4008Tg/hu4008Tg	amputation: caudal fin	Fig. 3. from Allanki et al., 2021
regenerating fin osteoblast EGFP expression decreased amount, abnormal	il11ra^{bns251/bns251}; hu4008Tg/hu4008Tg	amputation: caudal fin	Fig. 3. from Allanki et al., 2021
regenerating fin EGFP expression decreased amount, abnormal	il11ra^{bns251/bns251}; hu4008Tg/hu4008Tg	amputation: caudal fin	Fig. 3. from Allanki et al., 2021
caudal fin fin regeneration decreased process quality, abnormal	il11ra^{bns251/bns251}; hu4008Tg/hu4008Tg	amputation: caudal fin	Fig. 3. from Allanki et al., 2021
cardiac ventricle mesenchyme mylka expression increased amount, abnormal	il11ra^{bns251/bns251}; y1Tg/y1Tg	ablation: heart	Fig. 6. from Allanki et al., 2021
cardiac ventricle extracellular matrix secreting cell loxa expression increased amount, abnormal	il11ra^{bns251/bns251}; y1Tg/y1Tg	ablation: heart	Fig. 6. from Allanki et al., 2021
cardiac ventricle extracellular matrix secreting cell loxl2b expression increased amount, abnormal	il11ra^{bns251/bns251}; y1Tg/y1Tg	ablation: heart	Fig. 6. from Allanki et al., 2021
regenerating tissue blood vessel EGFP expression spatial pattern, abnormal	il11ra^{bns251/bns251}; y1Tg/y1Tg	amputation: caudal fin	Fig. 5. from Allanki et al., 2021
regenerating tissue endocardium EGFP expression increased amount, abnormal	il11ra^{bns251/bns251}; y1Tg/y1Tg	ablation: heart	Fig. 5. from Allanki et al., 2021
cardiac ventricle extracellular matrix secreting cell col1a1b expression increased amount, abnormal	il11ra^{bns251/bns251}; y1Tg/y1Tg	ablation: heart	Fig. 6. from Allanki et al., 2021
cardiac ventricle regeneration fibroblast Ab7-acta2 labeling spatial pattern, abnormal	il11ra^{bns251/bns251}; y1Tg/y1Tg	amputation: caudal fin	Fig. 5. from Allanki et al., 2021
regenerating tissue transforming growth factor beta production increased occurrence, abnormal	il11ra^{bns251/bns251}; y1Tg/y1Tg	ablation: heart	Fig. 8. from Allanki et al., 2021
cardiac ventricle regeneration fibroblast zns-5 labeling spatial pattern, abnormal	il11ra^{bns251/bns251}; y1Tg/y1Tg	amputation: caudal fin	Fig. 5. from Allanki et al., 2021
cardiac ventricle mesenchyme tgfb2 expression increased amount, abnormal	il11ra^{bns251/bns251}; y1Tg/y1Tg	ablation: heart	Fig. 6. from Allanki et al., 2021
regenerating tissue blood vessel EGFP expression increased amount, abnormal	il11ra^{bns251/bns251}; y1Tg/y1Tg	ablation: heart	Fig. 8. from Allanki et al., 2021
cardiac ventricle transforming growth factor beta receptor signaling pathway increased occurrence, abnormal	il11ra^{bns251/bns251}; y1Tg/y1Tg	ablation: heart	Fig. 8. from Allanki et al., 2021
endothelial cell tie1 expression increased amount, abnormal	il11ra^{bns251/bns251}; y1Tg/y1Tg	ablation: heart	Fig. 6. from Allanki et al., 2021
regenerating tissue endocardium increased amount, abnormal	il11ra^{bns251/bns251}; y1Tg/y1Tg	ablation: heart	Fig. 6. from Allanki et al., 2021
regenerating tissue endocardium ab1-elna labeling increased amount, abnormal	il11ra^{bns251/bns251}; y1Tg/y1Tg	ablation: heart	Fig. 5. from Allanki et al., 2021
cardiac ventricle extracellular matrix secreting cell elnb expression increased amount, abnormal	il11ra^{bns251/bns251}; y1Tg/y1Tg	ablation: heart	Fig. 6. from Allanki et al., 2021
cardiac ventricle mesenchyme acta2 expression increased amount, abnormal	il11ra^{bns251/bns251}; y1Tg/y1Tg	ablation: heart	Fig. 6. from Allanki et al., 2021
regenerating tissue endocardium ab2-smad3 labeling increased amount, abnormal	il11ra^{bns251/bns251}; y1Tg/y1Tg	ablation: heart	Fig. 8. from Allanki et al., 2021
cardiac ventricle mesenchyme snai1b expression increased amount, abnormal	il11ra^{bns251/bns251}; y1Tg/y1Tg	ablation: heart	Fig. 6. from Allanki et al., 2021
cardiac ventricle extracellular matrix secreting cell col1a1a expression increased amount, abnormal	il11ra^{bns251/bns251}; y1Tg/y1Tg	ablation: heart	Fig. 6. from Allanki et al., 2021
cardiac muscle cell substrate-dependent cell migration, cell extension decreased process quality, abnormal	il11ra^{bns251/bns251}; bns417Tg/bns417Tg; cn9Tg/cn9Tg	ablation: heart	Fig. 7. from Allanki et al., 2021
cardiac muscle cell substrate-dependent cell migration, cell extension decreased process quality, ameliorated	il11ra^{bns251/bns251}; bns417Tg/bns417Tg; cn9Tg/cn9Tg	ablation: heart, chemical treatment by environment: afimoxifene	Fig. 7. from Allanki et al., 2021
cardiac ventricle regeneration fibroblast Ab7-acta2 labeling increased amount, abnormal	il11ra^{bns251/bns251}; bns417Tg/bns417Tg; cn9Tg/cn9Tg	ablation: heart	Fig. 7. from Allanki et al., 2021
cardiac ventricle regeneration fibroblast Ab7-acta2 labeling amount, ameliorated	il11ra^{bns251/bns251}; bns417Tg/bns417Tg; cn9Tg/cn9Tg	ablation: heart, chemical treatment by environment: afimoxifene	Fig. 7. from Allanki et al., 2021

Citations