CRISPR

CRISPR2-tfam

ID

ZDB-CRISPR-230821-5

Name

CRISPR2-tfam

Previous Names

None

Target

Sequence

5' - TATGCTGTCAGGGGTCTCTT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
nu201	tfam

Expression

Gene expression in Wild Types + CRISPR2-tfam

No data available

Phenotype

Phenotype resulting from CRISPR2-tfam

Phenotype	Fish	Figures
whole organism mitochondrial chromosome decreased amount, abnormal	WT + CRISPR2-tfam	Fig. S6 from Ullah et al., 2021

Phenotype of all Fish created by or utilizing CRISPR2-tfam

Phenotype	Fish	Conditions	Figures
head mt-co1 expression decreased amount, abnormal	tfam^nu201/nu201	standard conditions	Fig. S5 from Ullah et al., 2021
caudal fin mitochondrial chromosome decreased amount, abnormal	tfam^nu201/nu201	standard conditions	Fig. S5 from Ullah et al., 2021
gonad cyp19a1a expression decreased amount, abnormal	tfam^nu201/nu201	standard conditions	Fig. 5 from Ullah et al., 2021
gonad tfam expression decreased amount, abnormal	tfam^nu201/nu201	standard conditions	Fig. 5 from Ullah et al., 2021
gonad amh expression decreased amount, abnormal	tfam^nu201/nu201	standard conditions	Fig. 5 from Ullah et al., 2021
whole organism sterile, abnormal	tfam^nu201/nu201	standard conditions	text only from Ullah et al., 2021
gonad ddx4 expression decreased amount, abnormal	tfam^nu201/nu201	standard conditions	Fig. 5 from Ullah et al., 2021
head mitochondrial chromosome decreased amount, abnormal	tfam^nu201/nu201	standard conditions	Fig. S5 from Ullah et al., 2021
head mt-nd1 expression decreased amount, abnormal	tfam^nu201/nu201	standard conditions	Fig. S5 from Ullah et al., 2021
whole organism decreased length, abnormal	tfam^nu201/nu201	standard conditions	Fig. S4 from Ullah et al., 2021
ovary oocyte decreased amount, abnormal	tfam^nu201/nu201	standard conditions	Fig. 5 from Ullah et al., 2021
whole organism mitochondrial chromosome decreased amount, abnormal	WT + CRISPR2-tfam	control	Fig. S6 from Ullah et al., 2021

Citations