CRISPR

CRISPR1-ankle2

ID
ZDB-CRISPR-230607-1
Name
CRISPR1-ankle2
Previous Names
None
Target
Sequence
5' - GCTCTAGTCGTGGCAGTGAT - 3'
ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
The PAM site was "GGG" at the 3' end.
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
oi5 ankle2
Expression
Gene expression in Wild Types + CRISPR1-ankle2
No data available
Phenotype
Phenotype resulting from CRISPR1-ankle2
No data available
Phenotype of all Fish created by or utilizing CRISPR1-ankle2
Phenotype Fish Conditions Figures
cerebellum decreased size, abnormal ankle2oi5/oi5 (AB) standard conditions Fig. 2 from Apridita Sebastian et al., 2022
dorsal telencephalon neural precursor cell proliferation decreased process quality, abnormal ankle2oi5/oi5 (AB) standard conditions Fig. 1,  Fig. 3 from Apridita Sebastian et al., 2022
testis decreased size, abnormal ankle2oi5/oi5 (AB) standard conditions Fig. 4 from Apridita Sebastian et al., 2022
dorsal telencephalon generation of neurons decreased process quality, abnormal ankle2oi5/oi5 (AB) standard conditions Fig. 1,  Fig. 2,  Fig. 3 from Apridita Sebastian et al., 2022
brain nuclear envelope organization decreased process quality, abnormal ankle2oi5/oi5 (AB) standard conditions Fig. 2,  Fig. 3 from Apridita Sebastian et al., 2022
brain decreased size, abnormal ankle2oi5/oi5 (AB) standard conditions Fig. 2,  Fig. 3 from Apridita Sebastian et al., 2022
telencephalon has fewer parts of type neuron, abnormal ankle2oi5/oi5 (AB) standard conditions Fig. 2 from Apridita Sebastian et al., 2022
head decreased size, abnormal ankle2oi5/oi5 (AB) standard conditions Fig. 1 from Apridita Sebastian et al., 2022
testis decreased thickness, abnormal ankle2oi5/oi5 (AB) standard conditions Fig. 4 from Apridita Sebastian et al., 2022
dorsal telencephalon has fewer parts of type radial glial cell, abnormal ankle2oi5/oi5 (AB) standard conditions Fig. 1,  Fig. 3 from Apridita Sebastian et al., 2022
testis lacks all parts of type sperm, abnormal ankle2oi5/oi5 (AB) standard conditions Fig. 4 from Apridita Sebastian et al., 2022
locomotion decreased process quality, abnormal ankle2oi5/oi5 (AB) standard conditions Fig. 1 from Apridita Sebastian et al., 2022
brain nuclear envelope malformed, abnormal ankle2oi5/oi5 (AB) standard conditions Fig. 2,  Fig. 3 from Apridita Sebastian et al., 2022
male organism male sterile, abnormal ankle2oi5/oi5 (AB) standard conditions Fig. 4 from Apridita Sebastian et al., 2022
telencephalon decreased size, abnormal ankle2oi5/oi5 (AB) standard conditions Fig. 2 from Apridita Sebastian et al., 2022
spermatogenesis arrested, abnormal ankle2oi5/oi5 (AB) standard conditions Fig. 4 from Apridita Sebastian et al., 2022
dorsal telencephalon generation of neurons process quality, ameliorated ankle2oi5/oi5 + MO1-vrk1 (AB) standard conditions Fig. 3 from Apridita Sebastian et al., 2022
dorsal telencephalon has number of radial glial cell, ameliorated ankle2oi5/oi5 + MO1-vrk1 (AB) standard conditions Fig. 3 from Apridita Sebastian et al., 2022
dorsal telencephalon neural precursor cell proliferation process quality, ameliorated ankle2oi5/oi5 + MO1-vrk1 (AB) standard conditions Fig. 3 from Apridita Sebastian et al., 2022
testis has fewer parts of type sperm, ameliorated ankle2oi5/oi5; vrk1oi6/+ (AB) standard conditions Fig. 4 from Apridita Sebastian et al., 2022
brain size, ameliorated ankle2oi5/oi5; vrk1oi6/+ (AB) standard conditions Fig. 3 from Apridita Sebastian et al., 2022
dorsal telencephalon generation of neurons process quality, ameliorated ankle2oi5/oi5; vrk1oi6/+ (AB) standard conditions Fig. 3 from Apridita Sebastian et al., 2022
dorsal telencephalon has number of radial glial cell, ameliorated ankle2oi5/oi5; vrk1oi6/+ (AB) standard conditions Fig. 3 from Apridita Sebastian et al., 2022
spermatogenesis occurrence, ameliorated ankle2oi5/oi5; vrk1oi6/+ (AB) standard conditions Fig. 4 from Apridita Sebastian et al., 2022
brain nuclear envelope morphology, ameliorated ankle2oi5/oi5; vrk1oi6/+ (AB) standard conditions Fig. 3 from Apridita Sebastian et al., 2022
male organism male sterile, abnormal ankle2oi5/oi5; vrk1oi6/+ (AB) standard conditions Fig. 4 from Apridita Sebastian et al., 2022
testis size, ameliorated ankle2oi5/oi5; vrk1oi6/+ (AB) standard conditions Fig. 4 from Apridita Sebastian et al., 2022
dorsal telencephalon neural precursor cell proliferation process quality, ameliorated ankle2oi5/oi5; vrk1oi6/+ (AB) standard conditions Fig. 3 from Apridita Sebastian et al., 2022
brain nuclear envelope organization process quality, ameliorated ankle2oi5/oi5; vrk1oi6/+ (AB) standard conditions Fig. 3 from Apridita Sebastian et al., 2022
Citations
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Please check the highlighted fields and try again.
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if additional information is required.
Oops. Something went wrong. Please try again later.