CRISPR

CRISPR4-myh7

ID

ZDB-CRISPR-230411-5

Name

CRISPR4-myh7

Previous Names

None

Target

myh7 (1)

Sequence

5' - GATCAAGAGTAAGCTCAAG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "TGG" at the 3' end.

Genome Resources

None

Target Location

View all 6 target locations

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
hn2Tg	myh7

Expression

Gene expression in Wild Types + CRISPR4-myh7

No data available

Phenotype

Phenotype resulting from CRISPR4-myh7

No data available

Phenotype of all Fish created by or utilizing CRISPR4-myh7

Phenotype	Fish	Conditions	Figures
atrium EGFP expression mislocalised, abnormal	myh7^hn2Tg/hn2Tg	chemical treatment by environment: amantadine, chemical treatment by environment: paracetamol	FIGURE 3 from Ouyang et al., 2022
whole organism pitx2 expression decreased amount, abnormal	myh7^hn2Tg/hn2Tg	chemical treatment by environment: amantadine, chemical treatment by environment: paracetamol	FIGURE 4 from Ouyang et al., 2022
lateral plate mesoderm spaw expression decreased amount, abnormal	myh7^hn2Tg/hn2Tg	chemical treatment by environment: amantadine, chemical treatment by environment: paracetamol	FIGURE 4 from Ouyang et al., 2022
cardiac ventricle EGFP expression spatial pattern, abnormal	myh7^hn2Tg/hn2Tg	chemical treatment by environment: amantadine, chemical treatment by environment: paracetamol	FIGURE 3 from Ouyang et al., 2022
presumptive cardiac ventricle primitive heart tube pitx2 expression decreased amount, abnormal	myh7^hn2Tg/hn2Tg	chemical treatment by environment: amantadine, chemical treatment by environment: paracetamol	FIGURE 4 from Ouyang et al., 2022
whole organism tbx20 expression decreased amount, abnormal	myh7^hn2Tg/hn2Tg	chemical treatment by environment: amantadine, chemical treatment by environment: paracetamol	FIGURE 4 from Ouyang et al., 2022
presumptive cardiac ventricle primitive heart tube lft2 expression decreased amount, abnormal	myh7^hn2Tg/hn2Tg	chemical treatment by environment: amantadine, chemical treatment by environment: paracetamol	FIGURE 4 from Ouyang et al., 2022
lateral plate mesoderm gata4 expression decreased amount, abnormal	myh7^hn2Tg/hn2Tg	chemical treatment by environment: amantadine, chemical treatment by environment: paracetamol	FIGURE 4 from Ouyang et al., 2022
lateral plate mesoderm lft2 expression decreased amount, abnormal	myh7^hn2Tg/hn2Tg	chemical treatment by environment: amantadine, chemical treatment by environment: paracetamol	FIGURE 4 from Ouyang et al., 2022
presumptive cardiac ventricle primitive heart tube spaw expression decreased amount, abnormal	myh7^hn2Tg/hn2Tg	chemical treatment by environment: amantadine, chemical treatment by environment: paracetamol	FIGURE 4 from Ouyang et al., 2022
whole organism lft1 expression decreased amount, abnormal	myh7^hn2Tg/hn2Tg	chemical treatment by environment: amantadine, chemical treatment by environment: paracetamol	FIGURE 4 from Ouyang et al., 2022
whole organism lft2 expression decreased amount, abnormal	myh7^hn2Tg/hn2Tg	chemical treatment by environment: amantadine, chemical treatment by environment: paracetamol	FIGURE 4 from Ouyang et al., 2022
presumptive cardiac ventricle primitive heart tube lft1 expression decreased amount, abnormal	myh7^hn2Tg/hn2Tg	chemical treatment by environment: amantadine, chemical treatment by environment: paracetamol	FIGURE 4 from Ouyang et al., 2022
lateral plate mesoderm lft1 expression decreased amount, abnormal	myh7^hn2Tg/hn2Tg	chemical treatment by environment: amantadine, chemical treatment by environment: paracetamol	FIGURE 4 from Ouyang et al., 2022
whole organism mef2ca expression decreased amount, abnormal	myh7^hn2Tg/hn2Tg	chemical treatment by environment: amantadine, chemical treatment by environment: paracetamol	FIGURE 4 from Ouyang et al., 2022
lateral plate mesoderm mef2ca expression decreased amount, abnormal	myh7^hn2Tg/hn2Tg	chemical treatment by environment: amantadine, chemical treatment by environment: paracetamol	FIGURE 4 from Ouyang et al., 2022
whole organism nkx2.5 expression decreased amount, abnormal	myh7^hn2Tg/hn2Tg	chemical treatment by environment: amantadine, chemical treatment by environment: paracetamol	FIGURE 4 from Ouyang et al., 2022
lateral plate mesoderm nkx2.5 expression decreased amount, abnormal	myh7^hn2Tg/hn2Tg	chemical treatment by environment: amantadine, chemical treatment by environment: paracetamol	FIGURE 4 from Ouyang et al., 2022
lateral plate mesoderm tbx20 expression decreased amount, abnormal	myh7^hn2Tg/hn2Tg	chemical treatment by environment: amantadine, chemical treatment by environment: paracetamol	FIGURE 4 from Ouyang et al., 2022
regulation of heart looping decreased process quality, abnormal	myh7^hn2Tg/hn2Tg	chemical treatment by environment: amantadine, chemical treatment by environment: paracetamol	FIGURE 3 from Ouyang et al., 2022
whole organism spaw expression decreased amount, abnormal	myh7^hn2Tg/hn2Tg	chemical treatment by environment: amantadine, chemical treatment by environment: paracetamol	FIGURE 4 from Ouyang et al., 2022
whole organism gata4 expression decreased amount, abnormal	myh7^hn2Tg/hn2Tg	chemical treatment by environment: amantadine, chemical treatment by environment: paracetamol	FIGURE 4 from Ouyang et al., 2022
lateral plate mesoderm pitx2 expression decreased amount, abnormal	myh7^hn2Tg/hn2Tg	chemical treatment by environment: amantadine, chemical treatment by environment: paracetamol	FIGURE 4 from Ouyang et al., 2022

Citations