CRISPR

CRISPR1-grin2ba

ID

ZDB-CRISPR-230323-1

Name

CRISPR1-grin2ba

Previous Names

None

Target

grin2ba (1)

Sequence

5' - CATGAACGCAGCCAGGTTAG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "CGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
sbu308	grin2ba
sbu309	grin2ba
sbu310	grin2ba

Expression

Gene expression in Wild Types + CRISPR1-grin2ba

No data available

Phenotype

Phenotype resulting from CRISPR1-grin2ba

No data available

Phenotype of all Fish created by or utilizing CRISPR1-grin2ba

Phenotype	Fish	Conditions	Figures
regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay increased process quality, abnormal	grin2ba^{sbu310/sbu310}	standard conditions	Fig. 6 from Zoodsma et al., 2022
whole organism grin2ba expression decreased amount, abnormal	grin2ba^sbu310/+	standard conditions	Fig. 6 from Zoodsma et al., 2022
social behavior decreased process quality, abnormal	grin2ba^{sbu310/sbu310}; grin2bb^{sbu311/sbu311}	standard conditions	Fig. 3 from Zoodsma et al., 2022
whole organism grin2da expression increased amount, abnormal	grin2ba^{sbu310/sbu310}; grin2bb^{sbu311/sbu311}	standard conditions	Fig. 6 from Zoodsma et al., 2022
whole organism grin2ab expression increased amount, abnormal	grin2ba^{sbu310/sbu310}; grin2bb^{sbu311/sbu311}	standard conditions	Fig. 6 from Zoodsma et al., 2022
regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay increased process quality, abnormal	grin2ba^{sbu310/sbu310}; grin2bb^{sbu311/sbu311}	standard conditions	Fig. 6 from Zoodsma et al., 2022
whole organism grin1a expression increased amount, abnormal	grin2ba^{sbu310/sbu310}; grin2bb^{sbu311/sbu311}	standard conditions	Fig. 6 from Zoodsma et al., 2022
whole organism grin1b expression increased amount, abnormal	grin2ba^{sbu310/sbu310}; grin2bb^{sbu311/sbu311}	standard conditions	Fig. 6 from Zoodsma et al., 2022
whole organism grin2db expression increased amount, abnormal	grin2ba^{sbu310/sbu310}; grin2bb^{sbu311/sbu311}	standard conditions	Fig. 6 from Zoodsma et al., 2022
ventral telencephalon anterior commissure GFP expression decreased amount, abnormal	grin2ba^{sbu310/sbu310}; grin2bb^{sbu311/sbu311}; nns9Tg/nns9Tg; ot1Tg/ot1Tg	standard conditions	Fig. 7 from Zoodsma et al., 2022
ventral telencephalon lateral forebrain bundle GFP expression decreased amount, abnormal	grin2ba^{sbu310/sbu310}; grin2bb^{sbu311/sbu311}; nns9Tg/nns9Tg; ot1Tg/ot1Tg	standard conditions	Fig. 7 from Zoodsma et al., 2022
ventral telencephalon modulation of inhibitory postsynaptic potential decreased process quality, abnormal	grin2ba^{sbu310/sbu310}; grin2bb^{sbu311/sbu311}; nns9Tg/nns9Tg; ot1Tg/ot1Tg	standard conditions	Fig. 7 from Zoodsma et al., 2022
ventral telencephalon anterior side GFP expression decreased amount, abnormal	grin2ba^{sbu310/sbu310}; grin2bb^{sbu311/sbu311}; nns9Tg/nns9Tg; ot1Tg/ot1Tg	standard conditions	Fig. 7 from Zoodsma et al., 2022

Citations