CRISPR

CRISPR3-mafba

ID

ZDB-CRISPR-221101-2

Name

CRISPR3-mafba

Previous Names

None

Target

mafba (1)

Sequence

5' - GCACGGGGTGCTGATAGGAG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
hzu17	mafba

Expression

Gene expression in Wild Types + CRISPR3-mafba

No data available

Phenotype

Phenotype resulting from CRISPR3-mafba

No data available

Phenotype of all Fish created by or utilizing CRISPR3-mafba

Phenotype	Fish	Conditions	Figures
keratinocyte progenitor cell apoptotic process decreased rate, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 5 from Chen et al., 2021
head mdm2 expression increased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 7 from Chen et al., 2021
inner ear ionocyte atp6v1aa expression decreased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 6 from Chen et al., 2021
head cdkn3 expression increased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 7 from Chen et al., 2021
head dhfr expression decreased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 7 from Chen et al., 2021
inner ear NaK ionocyte atp1b2b expression decreased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 6 from Chen et al., 2021
head ccne1 expression decreased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 7 from Chen et al., 2021
head cul3a expression increased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 7 from Chen et al., 2021
NaK ionocyte decreased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 6 from Chen et al., 2021
head cdk4 expression decreased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 7 from Chen et al., 2021
head ab4-h2afx labeling increased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 8 from Chen et al., 2021
head tp53 expression increased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 7 from Chen et al., 2021
otolith decreased area, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 2 from Chen et al., 2021
vH ionocyte decreased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 6 from Chen et al., 2021
head ab2-atr labeling increased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 8 from Chen et al., 2021
inner ear vH ionocyte Ab6-atp6v1a labeling decreased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 6 from Chen et al., 2021
startle response decreased process quality, abnormal	mafba^hzu17/hzu17	vibration	Figure 3 from Chen et al., 2021
head foxa1 expression decreased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 7 from Chen et al., 2021
epidermal stem cell apoptotic process decreased rate, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 5 from Chen et al., 2021
vH ionocyte apoptotic process increased rate, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 6 from Chen et al., 2021
head mycb expression decreased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 7 from Chen et al., 2021
epidermal stem cell ab1-tp63 labeling decreased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 4 , Figure 5 from Chen et al., 2021
head baxa expression increased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 7 from Chen et al., 2021
inner ear NaK ionocyte atp1b1a expression decreased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 6 from Chen et al., 2021
head cdkn1bb expression increased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 7 from Chen et al., 2021
head cdk2 expression decreased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 7 from Chen et al., 2021
head Ab2-mafba labeling decreased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 1 from Chen et al., 2021
ionocyte cell population proliferation decreased rate, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 4 from Chen et al., 2021
epidermal stem cell cell population proliferation decreased rate, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 4 from Chen et al., 2021
inner ear vH ionocyte atp6v1aa expression decreased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 6 from Chen et al., 2021
ionocyte progenitor cell foxi3a expression decreased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 4 from Chen et al., 2021
inner ear ionocyte atp1a1a.4 expression decreased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 6 from Chen et al., 2021
head mdm4 expression decreased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 7 from Chen et al., 2021
head ccnd1 expression decreased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 7 from Chen et al., 2021
head mafba expression decreased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 1 from Chen et al., 2021
head e2f1 expression decreased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 7 from Chen et al., 2021
head Ab4-atm labeling increased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 8 from Chen et al., 2021
otolith lumen increased area, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 2 from Chen et al., 2021
head cdkn1a expression increased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 7 from Chen et al., 2021
ionocyte progenitor cell dlc expression decreased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 4 from Chen et al., 2021
inner ear ionocyte atp1b2b expression decreased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 6 from Chen et al., 2021
head casp8 expression increased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 7 from Chen et al., 2021
NaK ionocyte apoptotic process increased rate, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 6 from Chen et al., 2021
ionocyte apoptotic process decreased rate, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 5 from Chen et al., 2021
head bbc3 expression increased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 7 from Chen et al., 2021
head rangap1a expression decreased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 7 from Chen et al., 2021
ionocyte apoptotic process increased rate, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 6 , Figure 8 from Chen et al., 2021
ionocyte progenitor cell foxi3b expression decreased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 4 from Chen et al., 2021
head bcl2a expression decreased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 7 from Chen et al., 2021
head tk1 expression decreased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 7 from Chen et al., 2021
head e2f3 expression decreased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 7 from Chen et al., 2021
head cdkn2a/b expression increased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 7 from Chen et al., 2021
inner ear ionocyte ab4-h2afx labeling increased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 8 from Chen et al., 2021
inner ear NaK ionocyte atp1a1a.4 expression decreased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 6 from Chen et al., 2021
head cul1a expression increased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 7 from Chen et al., 2021
head rb1 expression decreased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 7 from Chen et al., 2021
keratinocyte progenitor cell cell population proliferation decreased rate, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 4 from Chen et al., 2021
inner ear ionocyte atp1b1a expression decreased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 6 from Chen et al., 2021
head cdk6 expression decreased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 7 from Chen et al., 2021
head casp10 expression increased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 7 from Chen et al., 2021
cell cycle phase transition decreased process quality, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 7 from Chen et al., 2021
ionocyte progenitor cell ab2-dlc labeling decreased amount, abnormal	mafba^hzu17/hzu17	standard conditions	Figure 4 , Figure 5 from Chen et al., 2021
retinal rod cell EGFP expression decreased distribution, abnormal	mafba^hzu17/+; nrl^hzu16/hzu16; kj2Tg	standard conditions	Fig 8 from Liu et al., 2022
retinal rod cell decreased amount, abnormal	mafba^hzu17/+; nrl^hzu16/hzu16; kj2Tg	standard conditions	Fig 8 from Liu et al., 2022
retinal rod cell absence of anatomical entity, abnormal	mafba^hzu17/hzu17; nrl^hzu16/hzu16; kj2Tg	standard conditions	Fig 8 from Liu et al., 2022
retinal rod cell EGFP expression absent, abnormal	mafba^hzu17/hzu17; nrl^hzu16/hzu16; kj2Tg	standard conditions	Fig 8 from Liu et al., 2022
whole organism dead, abnormal	mafba^hzu17/hzu17; nrl^hzu16/hzu16; kj2Tg	standard conditions	text only from Liu et al., 2022

Citations