CRISPR

CRISPR5-asah1a

ID

ZDB-CRISPR-220923-2

Name

CRISPR5-asah1a

Previous Names

None

Target

asah1a (1)

Sequence

5' - GGTGTCCATCTCTCACTAGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The first "G" was added.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
zf3575	asah1a

Expression

Gene expression in Wild Types + CRISPR5-asah1a

No data available

Phenotype

Phenotype resulting from CRISPR5-asah1a

No data available

Phenotype of all Fish created by or utilizing CRISPR5-asah1a

Phenotype	Fish	Conditions	Figures
whole organism cholesteryl glycoside increased amount, abnormal	asah1a^{zf3575/zf3575} (AB, AB/TL)	chemical treatment by environment: EC 3.2.1.45 (glucosylceramidase) inhibitor	Fig. 3 from Lelieveld et al., 2022
whole organism cholesteryl glycoside increased amount, abnormal	asah1a^zf3575/+; asah1b^{zf3576/zf3576} (AB/TL)	chemical treatment by environment: EC 3.2.1.45 (glucosylceramidase) inhibitor	Fig. 3 from Lelieveld et al., 2022
whole organism HexCer(d34:1) increased amount, abnormal	asah1a^zf3575/+; asah1b^{zf3576/zf3576} (AB/TL)	chemical treatment by environment: EC 3.2.1.45 (glucosylceramidase) inhibitor	Fig. 3 from Lelieveld et al., 2022
whole organism ceramide increased amount, abnormal	asah1a^zf3575/+; asah1b^{zf3576/zf3576} (AB/TL)	control	Fig. 3 from Lelieveld et al., 2022
whole organism D-glucosylsphingosine increased amount, abnormal	asah1a^{zf3575/zf3575}; asah1b^zf3576/+ (AB/TL)	chemical treatment by environment: EC 3.2.1.45 (glucosylceramidase) inhibitor	Fig. 3 from Lelieveld et al., 2022
whole organism HexCer(d34:1) increased amount, abnormal	asah1a^{zf3575/zf3575}; asah1b^zf3576/+ (AB/TL)	chemical treatment by environment: EC 3.2.1.45 (glucosylceramidase) inhibitor	Fig. 3 from Lelieveld et al., 2022
whole organism cholesteryl glycoside increased amount, abnormal	asah1a^{zf3575/zf3575}; asah1b^{zf3576/zf3576} (AB/TL)	chemical treatment by environment: EC 3.2.1.45 (glucosylceramidase) inhibitor	Fig. 3 from Lelieveld et al., 2022
whole organism HexCer(d34:1) increased amount, abnormal	asah1a^{zf3575/zf3575}; asah1b^{zf3576/zf3576} (AB/TL)	chemical treatment by environment: EC 3.2.1.45 (glucosylceramidase) inhibitor	Fig. 3 from Lelieveld et al., 2022
whole organism ceramide increased amount, abnormal	asah1a^{zf3575/zf3575}; asah1b^{zf3576/zf3576} (AB/TL)	control	Fig. 3 from Lelieveld et al., 2022

Citations