CRISPR

CRISPR3-cep290

ID
ZDB-CRISPR-220916-3
Name
CRISPR3-cep290
Previous Names
None
Target
Sequence
5' - GGAAGGCCTTAGGGATCTGAC - 3'
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
The PAM site was "GGG" at the 3' end.
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
fb208 cep290
Expression
Gene expression in Wild Types + CRISPR3-cep290
No data available
Phenotype
Phenotype resulting from CRISPR3-cep290
No data available
Phenotype of all Fish created by or utilizing CRISPR3-cep290
Phenotype Fish Conditions Figures
eye unc119a2 expression increased amount, abnormal cep290fb208/fb208 (AB/TU) standard conditions Fig. 5. with image from Cardenas-Rodriguez et al., 2021
whole organism cep290 expression absent, abnormal cep290fb208/fb208 (AB/TU) standard conditions Fig. 1. with image from Cardenas-Rodriguez et al., 2021
kidney unc119a2 expression increased amount, abnormal cep290fb208/fb208 (AB/TU) standard conditions Fig. 6. with image from Cardenas-Rodriguez et al., 2021
axis curved, abnormal cep290fb208/fb208 (AB/TU) standard conditions Fig. 4. with image from Cardenas-Rodriguez et al., 2021
retina layer formation disrupted, abnormal cep290fb208/fb208 (AB/TU) standard conditions Fig. 2. with imageFig. 3. with image from Cardenas-Rodriguez et al., 2021
post-vent region curved, abnormal cep290fb208/fb208 (AB/TU) standard conditions Fig. 4. with image from Cardenas-Rodriguez et al., 2021
retinal outer plexiform layer cep290 expression absent, abnormal cep290fb208/fb208 (AB/TU) standard conditions Fig. 2. with image from Cardenas-Rodriguez et al., 2021
retinal inner plexiform layer cep290 expression absent, abnormal cep290fb208/fb208 (AB/TU) standard conditions Fig. 2. with image from Cardenas-Rodriguez et al., 2021
whole organism arl13b expression increased amount, abnormal cep290fb208/fb208 (AB/TU) standard conditions Fig. 5. with image from Cardenas-Rodriguez et al., 2021
eye arl3b expression increased amount, abnormal cep290fb208/fb208 (AB/TU) standard conditions Fig. 5. with image from Cardenas-Rodriguez et al., 2021
whole organism unc119a2 expression increased amount, abnormal cep290fb208/fb208 (AB/TU) standard conditions Fig. 5. with image from Cardenas-Rodriguez et al., 2021
eye arl13b expression increased amount, abnormal cep290fb208/fb208 (AB/TU) standard conditions Fig. 5. with image from Cardenas-Rodriguez et al., 2021
axis kinked, abnormal cep290fb208/fb208 (AB/TU) standard conditions Fig. 4. with image from Cardenas-Rodriguez et al., 2021
peripheral olfactory organ cilium cep290 expression absent, abnormal cep290fb208/fb208 (AB/TU) standard conditions Fig. 2. with image from Cardenas-Rodriguez et al., 2021
Kupffer's vesicle ciliary base cep290 expression absent, abnormal cep290fb208/fb208 (AB/TU) standard conditions Fig. 2. with image from Cardenas-Rodriguez et al., 2021
whole organism arl3b expression increased amount, abnormal cep290fb208/fb208 (AB/TU) standard conditions Fig. 5. with image from Cardenas-Rodriguez et al., 2021
post-vent region curved ventral, abnormal cep290fb208/fb208 (AB/TU) standard conditions Fig. 1. with image from Cardenas-Rodriguez et al., 2021
pronephros cilium cep290 expression absent, abnormal cep290fb208/fb208 (AB/TU) standard conditions Fig. 2. with image from Cardenas-Rodriguez et al., 2021
photoreceptor outer segment layer disorganized, abnormal cep290fb208/fb208 (AB/TU) standard conditions Fig. 3. with image from Cardenas-Rodriguez et al., 2021
axis curved ventral, abnormal cep290fb208/fb208 (AB/TU) standard conditions Fig. 1. with image from Cardenas-Rodriguez et al., 2021
photoreceptor outer segment layer photoreceptor connecting cilium accumulation photoreceptor outer segment layer vesicle, abnormal cep290fb208/fb208 (AB/TU) standard conditions Fig. 3. with image from Cardenas-Rodriguez et al., 2021
Citations