CRISPR

CRISPR1-abcc6b.2

ID

ZDB-CRISPR-220328-4

Name

CRISPR1-abcc6b.2

Previous Names

None

Target

Sequence

5' - AGAAGCATCCTCAACTGGACA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "TGG" at the 3' end. The first 4 nucleotides do not target the gene in the current build.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
elu16	abcc6b.2

Expression

Gene expression in Wild Types + CRISPR1-abcc6b.2

No data available

Phenotype

Phenotype resulting from CRISPR1-abcc6b.2

No data available

Phenotype of all Fish created by or utilizing CRISPR1-abcc6b.2

Phenotype	Fish	Conditions	Figures
vertebra bone mineralization increased occurrence, abnormal	abcc6a^elu15/elu15; abcc6b.2^elu16/+	standard conditions	FIGURE 2 from Czimer et al., 2021
vertebra calcified, abnormal	abcc6a^elu15/elu15; abcc6b.2^elu16/+	standard conditions	FIGURE 2 from Czimer et al., 2021
vertebra calcified, abnormal	abcc6a^elu15/elu15; abcc6b.2^elu16/elu16	standard conditions	FIGURE 2 , FIGURE 3 from Czimer et al., 2021
vertebra malformed, abnormal	abcc6a^elu15/elu15; abcc6b.2^elu16/elu16	standard conditions	FIGURE 3 from Czimer et al., 2021
vertebra bone mineralization increased occurrence, abnormal	abcc6a^elu15/elu15; abcc6b.2^elu16/elu16	standard conditions	FIGURE 2 , FIGURE 3 from Czimer et al., 2021
vertebral column morphology, abnormal	abcc6a^elu15/elu15; abcc6b.2^elu16/elu16	standard conditions	FIGURE 3 from Czimer et al., 2021
vertebral column kinked, abnormal	abcc6a^elu15/elu15; abcc6b.2^elu16/elu16	standard conditions	FIGURE 3 from Czimer et al., 2021

Citations