CRISPR

CRISPR2-tdp1

ID

ZDB-CRISPR-211001-2

Name

CRISPR2-tdp1

Previous Names

None

Target

tdp1 (1)

Sequence

5' - GGAAGAGAGAAACTGAGGAA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
sh475	tdp1
sh476	tdp1

Expression

Gene expression in Wild Types + CRISPR2-tdp1

No data available

Phenotype

Phenotype resulting from CRISPR2-tdp1

No data available

Phenotype of all Fish created by or utilizing CRISPR2-tdp1

Phenotype	Fish	Conditions	Figures
whole organism phosphodiesterase activity, acting on 3'-phosphoglycolate-terminated DNA strands decreased process quality, abnormal	tdp1^sh475/sh475	chemical treatment by environment: camptothecin	Fig. 5 from Zaksauskaite et al., 2021
whole organism mus81 expression decreased amount, abnormal	tdp1^sh475/sh475	chemical treatment by environment: camptothecin	Fig. 6 from Zaksauskaite et al., 2021
gut phosphodiesterase activity, acting on 3'-phosphoglycolate-terminated DNA strands decreased process quality, abnormal	tdp1^sh475/sh475	chemical treatment by injection: topotecan	Fig. 4 from Zaksauskaite et al., 2021
whole organism tdp2b expression decreased amount, abnormal	tdp1^sh475/sh475	control	Fig. 6 from Zaksauskaite et al., 2021
locomotory behavior decreased process quality, abnormal	tdp1^sh475/sh475	standard conditions	Fig. 3 , Fig. 4 from Zaksauskaite et al., 2021
whole organism ercc1 expression decreased amount, abnormal	tdp1^sh475/sh475	chemical treatment by environment: camptothecin	Fig. 6 from Zaksauskaite et al., 2021
whole organism tdp1 expression decreased amount, abnormal	tdp1^sh475/sh475	control	Fig. 6 from Zaksauskaite et al., 2021
whole organism apex2 expression increased amount, abnormal	tdp1^sh475/sh475	chemical treatment by environment: camptothecin	Fig. 6 from Zaksauskaite et al., 2021
locomotory behavior decreased process quality, abnormal	tdp1^sh475/sh475	chemical treatment by injection: topotecan	Fig. 4 from Zaksauskaite et al., 2021
whole organism phosphodiesterase activity, acting on 3'-phosphoglycolate-terminated DNA strands absent process, abnormal	tdp1^sh475/sh475	standard conditions	Fig. 2 from Zaksauskaite et al., 2021
whole organism tdp1 expression decreased amount, abnormal	tdp1^sh475/sh475	chemical treatment by environment: camptothecin	Fig. 6 from Zaksauskaite et al., 2021
whole organism ercc4 expression increased amount, abnormal	tdp1^sh475/sh475	chemical treatment by environment: camptothecin	Fig. 6 from Zaksauskaite et al., 2021
whole organism tdp2b expression amount, ameliorated	tdp1^sh475/sh475	chemical treatment by environment: camptothecin	Fig. 6 from Zaksauskaite et al., 2021
whole organism phosphodiesterase activity, acting on 3'-phosphoglycolate-terminated DNA strands absent process, abnormal	tdp1^sh475/+; tdp1^sh476/+	standard conditions	Fig. 2 from Zaksauskaite et al., 2021

Citations