CRISPR

CRISPR1-ca2

ID

ZDB-CRISPR-210907-2

Name

CRISPR1-ca2

Previous Names

None

Target

Sequence

5' - GGCGGAATGGCTGACCACTG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "GGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ot206	ca2

Expression

Gene expression in Wild Types + CRISPR1-ca2

No data available

Phenotype

Phenotype resulting from CRISPR1-ca2

No data available

Phenotype of all Fish created by or utilizing CRISPR1-ca2

Phenotype	Fish	Conditions	Figures
whole organism ca2 expression absent, abnormal	ca2^ot206/ot206	standard conditions	Fig. 1 from Zimmer et al., 2020
yolk syncytial layer ca2 expression absent, abnormal	ca2^ot206/ot206	standard conditions	Fig. 1 from Zimmer et al., 2020
whole organism sodium ion transport increased occurrence, abnormal	ca2^ot206/ot206	standard conditions	Fig. 3 from Zimmer et al., 2020
whole organism dead, abnormal	ca2^ot206/ot206	standard conditions	Fig. 2 from Zimmer et al., 2020
yolk syncytial layer proton transmembrane transport decreased occurrence, abnormal	ca2^ot206/ot206	standard conditions	Fig. 7 from Zimmer et al., 2020
NCC ionocyte decreased amount, abnormal	ca2^ot206/ot206	standard conditions	Fig. 6 from Zimmer et al., 2020
whole organism chloride transport decreased occurrence, abnormal	ca2^ot206/ot206	standard conditions	Fig. 4 from Zimmer et al., 2020
whole organism ca2 expression decreased amount, abnormal	ca2^ot206/+	standard conditions	Fig. 1 from Zimmer et al., 2020

Citations